pmiReport PU.1 3' UTR
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26530||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6470
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Gene/Insert namePU.1 3' UTR
SpeciesM. musculus (mouse)
Insert Size (bp)333
Entrez GeneSpi1 (a.k.a. Dis-1, Dis1, PU.1, Sfpi-1, Sfpi1, Spi-1, Tcfpu1, Tfpu.1)
/ Fusion Protein
- Luciferase (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer Luc-F
- 3′ sequencing primer M13pUC-fwd (Common Sequencing Primers)
The inserts put into this vector are downstream from luciferase. They create different 3' UTRs for luciferase.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pmiReport PU.1 3' UTR was a gift from David Baltimore (Addgene plasmid # 26530 ; http://n2t.net/addgene:26530 ; RRID:Addgene_26530)
For your References section:Sustained expression of microRNA-155 in hematopoietic stem cells causes a myeloproliferative disorder. O'Connell RM, Rao DS, Chaudhuri AA, Boldin MP, Taganov KD, Nicoll J, Paquette RL, Baltimore D. J Exp Med. 2008 Mar 17. 205(3):585-94. 10.1084/jem.20072108 PubMed 18299402