Addgene - FCbAIGW Plasmid Data

Plasmid 26669: FCbAIGW

Gene/insert name:
None
Fusion protein or tag:
IRES-EGFP
Terminal:
C terminal on backbone
Vector backbone:
FCbAIGW
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral
Backbone size (bp):
11067
Cloning site 5':
XbaI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
FUGW-Fwd (ATTACAGGGACAGCAGAGATCC) List of Sequencing Primers
3' sequencing primer:
EGFP-N
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl2
Growth temperature (℃):
37
Growth instructions:
Stbl2
High or low copy:
Unknown
Selectable markers, other:
EGFP
Sequence: View sequences (3)
Principal Investigator:
Gerardo Morfini
Terms and Licenses:
MTA

Comments:

The vector has neuron specific promoter with downstream IRES2-EGFP sequence which allow the fluorescence marker protein to be expressed in a lower level under the same promoter with the transgene.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26669" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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