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pCAGGS-NICD
(Plasmid #26891)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26891 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCAG
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NICD
  • Alt name
    Notch intracellular domain
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2500
  • Mutation
    E2299K
  • GenBank ID
    NM_008714.3
  • Entrez Gene
    Notch1 (a.k.a. 9930111A19Rik, Mis6, N1, Tan1, lin-12)
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • b-globin polyA (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS-NICD was a gift from Nicholas Gaiano (Addgene plasmid # 26891 ; http://n2t.net/addgene:26891 ; RRID:Addgene_26891)
  • For your References section:

    Notch3 signaling promotes radial glial/progenitor character in the mammalian telencephalon. Dang L, Yoon K, Wang M, Gaiano N. Dev Neurosci. 2006;28(1-2):58-69. 10.1159/000090753 PubMed 16508304