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AAV-CAG-ChR2-GFP
(Plasmid #26929)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26929 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    AAV with CAG promoter
  • Backbone manufacturer
    Scott Sternson
  • Backbone size w/o insert (bp) 5454
  • Vector type
    Mammalian Expression, AAV ; adeno-associated virus

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Use recombinase-free E. coli, grow at 30C
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ChR2
  • Alt name
    Channelrhodopsin-2
  • Species
    C. reinhardtii
  • Insert Size (bp)
    930
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-CAG-ChR2-GFP was a gift from Edward Boyden (Addgene plasmid # 26929)
  • For your References section:

    Millisecond-timescale, genetically targeted optical control of neural activity. Boyden ES, Zhang F, Bamberg E, Nagel G, Deisseroth K. Nat Neurosci. 2005 Sep . 8(9):1263-8. 10.1038/nn1525 PubMed 16116447