|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||28305||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerScott Sternson
- Backbone size w/o insert (bp) 6482
Vector typeMammalian Expression, AAV, Cre/Lox ; Adeno-associated virus
Growth in Bacteria
Growth instructionsUse recombinase-free E. coli (Stbl3, XL-10, Sure2 et.al), grow at 30C
Copy numberLow Copy
Alt namearchaerhodopsin TP009
SpeciesH. strain TP009
Insert Size (bp)744
- Promoter CAG
/ Fusion Protein
- tdTomato (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site BsrGI (not destroyed)
- 5′ sequencing primer cttctggcgtgtgaccgg (Common Sequencing Primers)
PLEASE CONTACT ED BOYDEN ([email protected]) FOR DETAILS ON THIS REAGENT AND FURTHER REAGENTS IN THIS LINE.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-FLEX-ArchT-tdTomato was a gift from Edward Boyden (Addgene plasmid # 28305)
For your References section:A high-light sensitivity optical neural silencer: development and application to optogenetic control of non-human primate cortex. Han X, Chow BY, Zhou H, Klapoetke NC, Chuong A, Rajimehr R, Yang A, Baratta MV, Winkle J, Desimone R, Boyden ES. Front Syst Neurosci. 2011;5:18. Epub 2011 Apr 13. 10.3389/fnsys.2011.00018 PubMed 21811444