Addgene - pAAV-FLEX-ArchT-GFP Plasmid Data

Plasmid 28307: pAAV-FLEX-ArchT-GFP

Gene/insert name:
ArchT-GFP
Alt name:
archaerhodopsin
Alt name:
archaerhodopsin TP009
Alt name:
ArchT
Insert size:
744
Species:
H. strain TP009
Fusion protein or tag:
GFP
Terminal:
C terminal on insert
Mutation:
N/A
Vector backbone:
AAV-FLEX
(Search Vector Database)
Backbone manufacturer:
Scott Sternson
Vector type:
Mammalian Expression, Cre/Lox ; Adeno-associated virus
Backbone size w/o insert (bp):
5769
Promoter:
CAG
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
gctctagagcctctgctaacc List of Sequencing Primers
3' sequencing primer:
gcagcgtatccacatagcg
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
30
Growth instructions:
Use recombinase-free E. coli (Stbl3, XL-10, Sure2 et.al), grow at 30C
High or low copy:
Low Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Edward Boyden
Terms and Licenses:
MTA

Comments:

PLEASE CONTACT ED BOYDEN (esb@media.mit.edu) FOR DETAILS ON THIS REAGENT AND FURTHER REAGENTS IN THIS LINE.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A high-light sensitivity optical neural silencer: development and application to optogenetic control of non-human primate cortex. Han et al (Front Syst Neurosci. 2011;5:18. Epub 2011 Apr 13. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 28307" in your Materials and Methods section.