Addgene - pLenti6/V5-DEST-SPAG5 Plasmid Data

Plasmid 31217: pLenti6/V5-DEST-SPAG5

Gene/insert name:
SPAG5
Insert size:
3582
Species:
H. sapiens (human)
GenBank ID:
BC000322
Entrez Gene:
SPAG5 (MAP126, DEEPEST, hMAP126)
Mutation:
Contains a one nucleotide deletion at C-term that shifts out-of-frame with V5 tag in pLent6 V5/DEST; alters last four residues (EFLS* >LNF*)
Vector backbone:
pLenti6/V5-DEST
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
7000
Cloning method:
Gateway Cloning
Cloning site 5':
attR1
Site destroyed during cloning:
Yes
Cloning site 3':
attR2
Site destroyed during cloning:
Yes
5' sequencing primer:
CMV Forward List of Sequencing Primers
3' sequencing primer:
V5 Reverse
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
30
Growth instructions:
Grow in STBL3 bacteria (Invitrogen) at lower temps (~30C) to prevent recombination and increase plasmid production.
High or low copy:
High Copy
Selectable markers:
Blasticidin
Person or lab that originally
cloned the gene/insert:
The entry clone used to create this plasmid was obatined from the human ORFeome (v5.1) collection made available by the Center for Cancer Systems Biology at the Dana-Farber Cancer Institute in Boston, MA.
Sequence: View sequences (1)
Principal Investigator:
Lynda Chin
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Proinvasion metastasis drivers in early-stage melanoma are oncogenes. Scott et al (Cancer Cell. 2011 Jul 12;20(1):92-103. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31217" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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