Grow in STBL3 bacteria (Invitrogen) at lower temps (~30C) to prevent recombination and increase plasmid production.
The entry clone used to create this plasmid was obatined from the human ORFeome (v5.1) collection made available by the Center for Cancer Systems Biology at the Dana-Farber Cancer Institute in Boston, MA.
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
31217" in your Materials and Methods section.