- Backbone size w/o insert (bp) 4100
Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)2200
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (destroyed during cloning)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
Cre reporter: from blue fluorescence to yellow fluorescence
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LCMV:ECFP(loxP)EYFP was a gift from Gerhart Ryffel (Addgene plasmid # 31306)
For your References section:Tagging muscle cell lineages in development and tail regeneration using Cre recombinase in transgenic Xenopus. Ryffel GU, Werdien D, Turan G, Gerhards A, Goosses S, Senkel S. Nucleic Acids Res. 2003 Apr 15. 31(8):e44. 10.1093/nar/gng044 PubMed 12682379
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.