Addgene - LCMV:ECFP(loxP)EYFP Plasmid Data

Plasmid 31306: LCMV:ECFP(loxP)EYFP

Gene/insert name:
ECFP(loxP)EYFP
Insert size:
2200
Vector backbone:
pCS2+
(Search Vector Database)
Vector type:
Mammalian Expression, Cre/Lox
Backbone size w/o insert (bp):
4100
Cloning site 5':
HindIII
Site destroyed during cloning:
Yes
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Gerhart Ryffel
Terms and Licenses:
MTA

Comments:

Cre reporter: from blue fluorescence to yellow fluorescence

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Tagging muscle cell lineages in development and tail regeneration using Cre recombinase in transgenic Xenopus. Ryffel et al (Nucleic Acids Res. 2003 Apr 15. 31(8):e44. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31306" in your Materials and Methods section.