Plasmid 33273: pMXs-mGLIS1
  • Retroviral expression of mouse Glis1

  • GLIS1, Gateway casette A

  • RP23-399H3.1, Gli5, Gli6, GliH1

  • 2438

  • M. musculus (mouse)

  • 230587

  • Glis1 (RP23-399H3.1, Gli5, Gli6, GliH1)

  • pMXs
    (Search Vector Database)

  • Dr. Toshio Kitamura

  • Mammalian Expression, Retroviral

  • 4700

  • Gateway Cloning

  • pMX-S1811 List of Sequencing Primers

  • pMX-AS3200

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H. Gateway cassette is from Invitrogen Corporation.

  • View sequences (2)
  • View map

  • Shinya Yamanaka

    Kyoto University Ancillary Agreement

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Direct reprogramming of somatic cells is promoted by maternal transcription factor Glis1. Maekawa et al (Nature. 2011 . 474(7350):225-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 33273" in your Materials and Methods section.