Addgene - pMXs-mGLIS1 Plasmid Data

Plasmid 33273: pMXs-mGLIS1

Gene/insert name:
GLIS1, Gateway casette A
Alt name:
RP23-399H3.1, Gli5, Gli6, GliH1
Insert size:
2438
Species:
M. musculus (mouse)
GenBank ID:
230587
Entrez Gene:
Glis1 (RP23-399H3.1, Gli5, Gli6, GliH1)
Vector backbone:
pMXs
(Search Vector Database)
Backbone manufacturer:
Dr. Toshio Kitamura
Vector type:
Retroviral
Backbone size w/o insert (bp):
4700
Cloning method:
Gateway Cloning
5' sequencing primer:
pMX-S1811 List of Sequencing Primers
3' sequencing primer:
pMX-AS3200
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H. Gateway cassette is from Invitrogen Corporation.
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Shinya Yamanaka
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Direct reprogramming of somatic cells is promoted by maternal transcription factor Glis1. Maekawa et al (Nature. 2011 . 474(7350):225-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 33273" in your Materials and Methods section.