Addgene - pMIR-Report-Luc-KLF4-K5S Plasmid Data

Plasmid 34598: pMIR-Report-Luc-KLF4-K5S

Gene/insert name:
KLF4
Alt name:
GKLF
Alt name:
EZF
Insert size:
306
Species:
H. sapiens (human)
GenBank ID:
AF105036
Entrez Gene:
KLF4 (RP11-150J11.1, EZF, GKLF)
Vector backbone:
pMIR-Report-LUC
(Search Vector Database)
Backbone manufacturer:
Ambion
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
6470
Promoter:
CMV
Cloning site 5':
PmeI
Site destroyed during cloning:
Yes
Cloning site 3':
PmeI
Site destroyed during cloning:
Yes
5' sequencing primer:
use KLF4 or firefly luciferase internal primers List of Sequencing Primers
3' sequencing primer:
use KLF4 or firefly luciferase internal primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
XL1 Blue
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Michael Ruppert
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

This is a translation reporter. KLF4 cDNA (an 306 bp EcoRI fragment derived from the KLF4 3' UTR) was cloned into the PmeI site in the 3'UTR of the firefly luciferase transgene in pMIR-Report-Luciferase. The insert/cDNA fragment was generated by cleavage at KLF4-internal EcoRI sites within the KLF4 3' UTR, followed by Klenow treatment.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Lin et al (Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 34598" in your Materials and Methods section.