Addgene - pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry Plasmid Data

Plasmid 35498: pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry

Gene/insert name:
ChR1-VChR1 Chimera
Alt name:
C1V1 (t/t)
Insert size:
1809
Species:
Synthetic
GenBank ID:
AEL28923.1
Fusion protein or tag:
mCherry
Terminal:
C terminal on insert
Mutation:
E122T and E162T
Vector backbone:
pAAV
(Search Vector Database)
Vector type:
Mammalian Expression ; AAV
Backbone size w/o insert (bp):
5590
Modifications to Backbone:
Addition of Ef1a promoter, Lox sites and a WPRE
Promoter:
Ef1a
Cloning site 5':
AscI
Site destroyed during cloning:
No
Cloning site 3':
NheI
Site destroyed during cloning:
No
5' sequencing primer:
CACCCACACAAAGGAAAAGGGCC List of Sequencing Primers
3' sequencing primer:
GCAATAGCATGATACAAAGG
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (4)
Map:
View map
Principal Investigator:
Karl Deisseroth
Terms and Licenses:
MTA
Clontech Limited Use Label License

Comments:

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Neocortical excitation/inhibition balance in information processing and social dysfunction. Yizhar et al (Nature. 2011 Jul 27;477(7363):171-8. doi: 10.1038/nature10360. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35498" in your Materials and Methods section.