Addgene - TBRE-TK-Luc-WT Plasmid Data

Plasmid 36246: TBRE-TK-Luc-WT

Gene/insert name:
Two copies of the Brachyury T-box site
Fusion protein or tag:
Luciferase
Terminal:
C terminal on backbone
Vector backbone:
pTK-luc
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
6800
Promoter:
TK minimal promoter
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
N/A List of Sequencing Primers
3' sequencing primer:
Luc_N_Rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Supplemental document:
pTK-luc (application/pdf)
Principal Investigator:
Bruce Blumberg
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

Brachyury palindromic element:
AATTTCACACCTAGGTGTGAAATT

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: RIPPLY3 is a retinoic acid-inducible repressor required for setting the borders of the pre-placodal ectoderm. Janesick et al (Development. 2012 Mar;139(6):1213-24. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36246" in your Materials and Methods section.