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pAAV-CaMKIIa-eArchT3.0-P2A-EGFP-WPRE-hGHpA
(Plasmid #51110)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51110 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV-CaMKIIa-WPRE-HGHpA
  • Backbone manufacturer
    Karl Deisseroth
  • Backbone size w/o insert (bp) 6064
  • Total vector size (bp) 7034
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eArchT3.0
  • Alt name
    ArchT-TS-ERexport
  • Species
    Halorubrum sp. TP009
  • Insert Size (bp)
    970
  • Promoter CaMKIIa
  • Tag / Fusion Protein
    • P2A-EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site HpaI (not destroyed)
  • 5′ sequencing primer aggagcacgggcaggcgagtgg
  • 3′ sequencing primer CCATACGGGAAGCAATAGCATG
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This is a cell filling variant for expression of eArchT3.0 and cytosolic EGFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CaMKIIa-eArchT3.0-P2A-EGFP-WPRE-hGHpA was a gift from Jonathan Ting (Addgene plasmid # 51110)