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BirA(R118G)-HA destination vector
(Plasmid #53581)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53581 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1 MCS-BirA(R118G)-HA
  • Backbone manufacturer
    Kyle Roux, Addgene Plasmid 36047
  • Modifications to backbone
    pcDNA3.1 MCS-BirA(R118G)-HA was cut with HpaI and ligated to a blunt ended selection cassette (Gateway conversion kit, cassette B) containing AttR sites flanking the ccdB gene and the chloramphenicol resistance gene.
  • Vector type
    Mammalian Expression
  • Promoter CMV
  • Selectable markers
    Neomycin (select with G418)
  • Tags / Fusion Proteins
    • BirA(R118G) (C terminal on insert)
    • HA tag (C terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BirA(R118G)-HA destination vector was a gift from Karl Kramer (Addgene plasmid # 53581 ; http://n2t.net/addgene:53581 ; RRID:Addgene_53581)