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dCAS9-VP64_GFP Citations (41)

Plasmid Article: Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.
Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F Nature. 2014 Dec 10. doi: 10.1038/nature14136.
PubMed Journal

Articles Citing dCAS9-VP64_GFP

Engineering Synthetic Gene Circuits in Living Cells with CRISPR Technology. Jusiak B, Cleto S, Perez-Pinera P, Lu TK. Trends Biotechnol. 2016 Jan 22. pii: S0167-7799(15)00274-7. doi: 10.1016/j.tibtech.2015.12.014. PubMed
Manipulating the Prion Protein Gene Sequence and Expression Levels with CRISPR/Cas9. Kaczmarczyk L, Mende Y, Zevnik B, Jackson WS. PLoS One. 2016 Apr 29;11(4):e0154604. doi: 10.1371/journal.pone.0154604. eCollection 2016. PubMed

Associated Plasmids

Targeted HIV-1 Latency Reversal Using CRISPR/Cas9-Derived Transcriptional Activator Systems. Bialek JK, Dunay GA, Voges M, Schafer C, Spohn M, Stucka R, Hauber J, Lange UC. PLoS One. 2016 Jun 24;11(6):e0158294. doi: 10.1371/journal.pone.0158294. eCollection 2016. PubMed
Inhibition of histone deacetylase reduces transcription of NADPH oxidases and ROS production and ameliorates pulmonary arterial hypertension. Chen F, Li X, Aquadro E, Haigh S, Zhou J, Stepp DW, Weintraub NL, Barman SA, Fulton DJ. Free Radic Biol Med. 2016 Oct;99:167-178. doi: 10.1016/j.freeradbiomed.2016.08.003. Epub 2016 Aug 3. PubMed
Epigenetic Regulation of Interleukin 6 by Histone Acetylation in Macrophages and Its Role in Paraquat-Induced Pulmonary Fibrosis. Hu L, Yu Y, Huang H, Fan H, Hu L, Yin C, Li K, Fulton DJ, Chen F. Front Immunol. 2017 Jan 30;7:696. doi: 10.3389/fimmu.2016.00696. eCollection 2016. PubMed
Rational design of inducible CRISPR guide RNAs for de novo assembly of transcriptional programs. Ferry QR, Lyutova R, Fulga TA. Nat Commun. 2017 Mar 3;8:14633. doi: 10.1038/ncomms14633. PubMed
Wnt/Tcf1 pathway restricts embryonic stem cell cycle through activation of the Ink4/Arf locus. De Jaime-Soguero A, Aulicino F, Ertaylan G, Griego A, Cerrato A, Tallam A, Del Sol A, Cosma MP, Lluis F. PLoS Genet. 2017 Mar 27;13(3):e1006682. doi: 10.1371/journal.pgen.1006682. eCollection 2017 Mar. PubMed
RNA-guided transcriptional activation via CRISPR/dCas9 mimics overexpression phenotypes in Arabidopsis. Park JJ, Dempewolf E, Zhang W, Wang ZY. PLoS One. 2017 Jun 16;12(6):e0179410. doi: 10.1371/journal.pone.0179410. eCollection 2017. PubMed
An all-in-one UniSam vector system for efficient gene activation. Fidanza A, Lopez-Yrigoyen M, Romano N, Jones R, Taylor AH, Forrester LM. Sci Rep. 2017 Jul 25;7(1):6394. doi: 10.1038/s41598-017-06468-6. PubMed
Discovery of stimulation-responsive immune enhancers with CRISPR activation. Simeonov DR, Gowen BG, Boontanrart M, Roth TL, Gagnon JD, Mumbach MR, Satpathy AT, Lee Y, Bray NL, Chan AY, Lituiev DS, Nguyen ML, Gate RE, Subramaniam M, Li Z, Woo JM, Mitros T, Ray GJ, Curie GL, Naddaf N, Chu JS, Ma H, Boyer E, Van Gool F, Huang H, Liu R, Tobin VR, Schumann K, Daly MJ, Farh KK, Ansel KM, Ye CJ, Greenleaf WJ, Anderson MS, Bluestone JA, Chang HY, Corn JE, Marson A. Nature. 2017 Sep 7;549(7670):111-115. doi: 10.1038/nature23875. Epub 2017 Aug 30. PubMed

Associated Plasmids

CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation. Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto K, Sato M. Nat Methods. 2017 Oct;14(10):963-966. doi: 10.1038/nmeth.4430. Epub 2017 Sep 11. PubMed
Enhancer connectome in primary human cells identifies target genes of disease-associated DNA elements. Mumbach MR, Satpathy AT, Boyle EA, Dai C, Gowen BG, Cho SW, Nguyen ML, Rubin AJ, Granja JM, Kazane KR, Wei Y, Nguyen T, Greenside PG, Corces MR, Tycko J, Simeonov DR, Suliman N, Li R, Xu J, Flynn RA, Kundaje A, Khavari PA, Marson A, Corn JE, Quertermous T, Greenleaf WJ, Chang HY. Nat Genet. 2017 Nov;49(11):1602-1612. doi: 10.1038/ng.3963. Epub 2017 Sep 25. PubMed
Evaluating different DNA binding domains to modulate L1 ORF2p-driven site-specific retrotransposition events in human cells. Ade CM, Derbes RS, Wagstaff BJ, Linker SB, White TB, Deharo D, Belancio VP, Ivics Z, Roy-Engel AM. Gene. 2018 Feb 5;642:188-198. doi: 10.1016/j.gene.2017.11.033. Epub 2017 Nov 14. PubMed
Activation of the Anti-Aging and Cognition-Enhancing Gene Klotho by CRISPR-dCas9 Transcriptional Effector Complex. Chen CD, Zeldich E, Li Y, Yuste A, Abraham CR. J Mol Neurosci. 2018 Feb;64(2):175-184. doi: 10.1007/s12031-017-1011-0. Epub 2018 Jan 19. PubMed
Pinpointing recurrent proviral integration sites in new models for latent HIV-1 infection. Lange UC, Bialek JK, Walther T, Hauber J. Virus Res. 2018 Apr 2;249:69-75. doi: 10.1016/j.virusres.2018.03.007. Epub 2018 Mar 14. PubMed
CRISPR/Cas9-Based Cellular Engineering for Targeted Gene Overexpression. Osborn MJ, Lees CJ, McElroy AN, Merkel SC, Eide CR, Mathews W, Feser CJ, Tschann M, McElmury RT, Webber BR, Kim CJ, Blazar BR, Tolar J. Int J Mol Sci. 2018 Mar 22;19(4). pii: ijms19040946. doi: 10.3390/ijms19040946. PubMed
Mouse medulloblastoma driven by CRISPR activation of cellular Myc. Vo BT, Kwon JA, Li C, Finkelstein D, Xu B, Orr BA, Sherr CJ, Roussel MF. Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1038/s41598-018-24956-1. PubMed

Associated Plasmids

lncRNA PAPAS tethered to the rDNA enhancer recruits hypophosphorylated CHD4/NuRD to repress rRNA synthesis at elevated temperatures. Zhao Z, Senturk N, Song C, Grummt I. Genes Dev. 2018 Jun 1;32(11-12):836-848. doi: 10.1101/gad.311688.118. Epub 2018 Jun 15. PubMed
CRISPRa-mediated NEAT1 lncRNA upregulation induces formation of intact paraspeckles. Yamazaki T, Fujikawa C, Kubota A, Takahashi A, Hirose T. Biochem Biophys Res Commun. 2018 Sep 26;504(1):218-224. doi: 10.1016/j.bbrc.2018.08.158. Epub 2018 Sep 1. PubMed
Enhanced intrinsic CYP3A4 activity in human hepatic C3A cells with optically controlled CRISPR/dCas9 activator complex. Han S, Wei S, Wang X, Han X, Zhang M, Su M, Li Y, Guo J, Zeng W, Liu J, Gao Y, Shen L. Integr Biol (Camb). 2018 Dec 19;10(12):780-790. doi: 10.1039/c8ib00109j. PubMed
Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression. Knapp DJHF, Michaels YS, Jamilly M, Ferry QRV, Barbosa H, Milne TA, Fulga TA. Nat Commun. 2019 Apr 2;10(1):1490. doi: 10.1038/s41467-019-09148-3. PubMed
PI3K Inhibition Activates SGK1 via a Feedback Loop to Promote Chromatin-Based Regulation of ER-Dependent Gene Expression. Toska E, Castel P, Chhangawala S, Arruabarrena-Aristorena A, Chan C, Hristidis VC, Cocco E, Sallaku M, Xu G, Park J, Minuesa G, Shifman SG, Socci ND, Koche R, Leslie CS, Scaltriti M, Baselga J. Cell Rep. 2019 Apr 2;27(1):294-306.e5. doi: 10.1016/j.celrep.2019.02.111. PubMed
Cell-specific CRISPR-Cas9 activation by microRNA-dependent expression of anti-CRISPR proteins. Hoffmann MD, Aschenbrenner S, Grosse S, Rapti K, Domenger C, Fakhiri J, Mastel M, Borner K, Eils R, Grimm D, Niopek D. Nucleic Acids Res. 2019 Apr 15. pii: 5458120. doi: 10.1093/nar/gkz271. PubMed

Associated Plasmids

Targeted Transgene Activation in the Brain Tissue by Systemic Delivery of Engineered AAV1 Expressing CRISPRa. Lau CH, Ho JW, Lo PK, Tin C. Mol Ther Nucleic Acids. 2019 Jun 7;16:637-649. doi: 10.1016/j.omtn.2019.04.015. Epub 2019 Apr 23. PubMed

Associated Plasmids

Identification of Renal Long Non-coding RNA RP11-2B6.2 as a Positive Regulator of Type I Interferon Signaling Pathway in Lupus Nephritis. Liao Z, Ye Z, Xue Z, Wu L, Ouyang Y, Yao C, Cui C, Xu N, Ma J, Hou G, Wang J, Meng Y, Yin Z, Liu Y, Qian J, Zhang C, Ding H, Guo Q, Qu B, Shen N. Front Immunol. 2019 May 3;10:975. doi: 10.3389/fimmu.2019.00975. eCollection 2019. PubMed
Human evolved regulatory elements modulate genes involved in cortical expansion and neurodevelopmental disease susceptibility. Won H, Huang J, Opland CK, Hartl CL, Geschwind DH. Nat Commun. 2019 Jun 3;10(1):2396. doi: 10.1038/s41467-019-10248-3. PubMed
Regulation of CHD2 expression by the Chaserr long noncoding RNA gene is essential for viability. Rom A, Melamed L, Gil N, Goldrich MJ, Kadir R, Golan M, Biton I, Perry RB, Ulitsky I. Nat Commun. 2019 Nov 8;10(1):5092. doi: 10.1038/s41467-019-13075-8. PubMed
TUG1 Is a Regulator of AFP and Serves as Prognostic Marker in Non-Hepatitis B Non-Hepatitis C Hepatocellular Carcinoma. Lin YH, Wu MH, Huang YH, Yeh CT, Lin KH. Cells. 2020 Jan 21;9(2). pii: cells9020262. doi: 10.3390/cells9020262. PubMed
Chromosome 19 microRNA cluster enhances cell reprogramming by inhibiting epithelial-to-mesenchymal transition. Mong EF, Yang Y, Akat KM, Canfield J, VanWye J, Lockhart J, Tsibris JCM, Schatz F, Lockwood CJ, Tuschl T, Kayisli UA, Totary-Jain H. Sci Rep. 2020 Feb 20;10(1):3029. doi: 10.1038/s41598-020-59812-8. PubMed
Prioritizing disease and trait causal variants at the TNFAIP3 locus using functional and genomic features. Ray JP, de Boer CG, Fulco CP, Lareau CA, Kanai M, Ulirsch JC, Tewhey R, Ludwig LS, Reilly SK, Bergman DT, Engreitz JM, Issner R, Finucane HK, Lander ES, Regev A, Hacohen N. Nat Commun. 2020 Mar 6;11(1):1237. doi: 10.1038/s41467-020-15022-4. PubMed
A distal enhancer at risk locus 11q13.5 promotes suppression of colitis by Treg cells. Nasrallah R, Imianowski CJ, Bossini-Castillo L, Grant FM, Dogan M, Placek L, Kozhaya L, Kuo P, Sadiyah F, Whiteside SK, Mumbach MR, Glinos D, Vardaka P, Whyte CE, Lozano T, Fujita T, Fujii H, Liston A, Andrews S, Cozzani A, Yang J, Mitra S, Lugli E, Chang HY, Unutmaz D, Trynka G, Roychoudhuri R. Nature. 2020 Jul;583(7816):447-452. doi: 10.1038/s41586-020-2296-7. Epub 2020 May 13. PubMed
Enhancer RNAs predict enhancer-gene regulatory links and are critical for enhancer function in neuronal systems. Carullo NVN, Phillips Iii RA, Simon RC, Soto SAR, Hinds JE, Salisbury AJ, Revanna JS, Bunner KD, Ianov L, Sultan FA, Savell KE, Gersbach CA, Day JJ. Nucleic Acids Res. 2020 Aug 18. pii: 5893972. doi: 10.1093/nar/gkaa671. PubMed

Associated Plasmids

Controlling the Activity of CRISPR Transcriptional Regulators with Inducible sgRNAs. Ferry QRV, Fulga TA. Methods Mol Biol. 2021;2162:153-184. doi: 10.1007/978-1-0716-0687-2_9. PubMed
Identification of human long noncoding RNAs associated with nonalcoholic fatty liver disease and metabolic homeostasis. Ruan X, Li P, Ma Y, Jiang CF, Chen Y, Shi Y, Gupta N, Seifuddin F, Pirooznia M, Ohnishi Y, Yoneda N, Nishiwaki M, Dumbovic G, Rinn JL, Higuchi Y, Kawai K, Suemizu H, Cao H. J Clin Invest. 2021 Jan 4;131(1). pii: 136336. doi: 10.1172/JCI136336. PubMed
Identification and characterization of a new isoform of small GTPase RhoE. Dai Y, Luo W, Yue X, Ma W, Wang J, Chang J. Commun Biol. 2020 Oct 15;3(1):572. doi: 10.1038/s42003-020-01295-4. PubMed
Transcriptional behavior of the HIV-1 promoter in context of the BACH2 prominent proviral integration gene. Hamann MV, Ehmele P, Verdikt R, Bialek-Waldmann JK, Virdi S, Gunther T, Van Lint C, Grundhoff A, Hauber J, Lange UC. Virus Res. 2021 Feb;293:198260. doi: 10.1016/j.virusres.2020.198260. Epub 2020 Dec 11. PubMed
HIV-based lentiviral vectors: origin and sequence differences. Johnson NM, Alvarado AF, Moffatt TN, Edavettal JM, Swaminathan TA, Braun SE. Mol Ther Methods Clin Dev. 2021 Mar 27;21:451-465. doi: 10.1016/j.omtm.2021.03.018. eCollection 2021 Jun 11. PubMed
Titan Cells and Yeast Forms of Cryptococcus neoformans and Cryptococcus gattii Are Recognized by GXMR-CAR. Dos Santos MH, Machado MP, Kumaresan PR, da Silva TA. Microorganisms. 2021 Sep 5;9(9). pii: microorganisms9091886. doi: 10.3390/microorganisms9091886. PubMed
Salicylic acid inducible nucleocytoplasmic shuttling of NPR1 fusion proteins in human cells. Sadeghi F, Kumar M, Bandey IN, Li X, Roysam B, Varadarajan N. Biotechnol Bioeng. 2022 Jan;119(1):199-210. doi: 10.1002/bit.27966. Epub 2021 Nov 3. PubMed
Genome-wide CRISPR interference screen identifies long non-coding RNA loci required for differentiation and pluripotency. Haswell JR, Mattioli K, Gerhardinger C, Maass PG, Foster DJ, Peinado P, Wang X, Medina PP, Rinn JL, Slack FJ. PLoS One. 2021 Nov 3;16(11):e0252848. doi: 10.1371/journal.pone.0252848. eCollection 2021. PubMed
Homeoprotein SIX1 compromises antitumor immunity through TGF-beta-mediated regulation of collagens. Liu W, Gao M, Li L, Chen Y, Fan H, Cai Q, Shi Y, Pan C, Liu J, Cheng LS, Yang H, Cheng G. Cell Mol Immunol. 2021 Dec;18(12):2660-2672. doi: 10.1038/s41423-021-00800-x. Epub 2021 Nov 15. PubMed

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