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pLenti-sgRNA
(Plasmid #71409)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71409 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLenti-CRISPR
  • Backbone manufacturer
    Feng Zhang
  • Backbone size (bp) 9100
  • Modifications to backbone
    removed Cas9 from Feng Zhang's pLentiCRISPR plasmid
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

It is a lentiviral plasmid so it may be recommended to be propagated at 30oc but we typically grow at 37oC.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-sgRNA was a gift from Eric Lander & David Sabatini (Addgene plasmid # 71409 ; http://n2t.net/addgene:71409 ; RRID:Addgene_71409)