Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8388||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4800
Vector typeMammalian Expression, Cre/Lox ; Beta-gal
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namefloxed EGFP beta-gal
Alt nameloxI EGFP loxII
Insert Size (bp)4400
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (destroyed during cloning)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
Terms and Licenses
StuI/SalI fragment from pSV-EGFP/beta-gal cloned into XhoI site of pCAGGS. Floxed EGFP gene switch cassette driven by CMV enhancer/chicken beta-actin promoter. Floxed EGFP cassette can be removed using KpnI.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p169 pCMV/betaAc-EGFP/beta-gal was a gift from Jeffrey Green (Addgene plasmid # 8388 ; http://n2t.net/addgene:8388 ; RRID:Addgene_8388)
For your References section:A single vector containing modified cre recombinase and LOX recombination sequences for inducible tissue-specific amplification of gene expression. Kaczmarczyk SJ, Green JE. Nucleic Acids Res 2001 Jun 15;29(12):E56-6. 10.1093/nar/29.12.e56 PubMed 11410679
Map uploaded by the depositor.