Addgene - p169 pCMV/betaAc-EGFP/beta-gal Plasmid Data

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Plasmid 8388: p169 pCMV/betaAc-EGFP/beta-gal

Gene/insert name:
floxed EGFP beta-gal
Alt name:
loxI EGFP loxII
Alt name:
beta-gal
Insert size:
4400
Vector backbone:
pCAGGS
(Search Vector Database)
Vector type:
Mammalian Expression, Cre/Lox
Vector type:
Beta-gal
Backbone size w/o insert:
4800
Cloning site 5':
XhoI
Site destroyed during cloning:
Yes
Cloning site 3':
XhoI
Site destroyed during cloning:
Yes
5' sequencing primer:
pCAG-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Jeffrey Green
Terms and Licenses:
MTA

Comments:

StuI/SalI fragment from pSV-EGFP/beta-gal cloned into XhoI site of pCAGGS. Floxed EGFP gene switch cassette driven by CMV enhancer/chicken beta-actin promoter. Floxed EGFP cassette can be removed using KpnI.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A single vector containing modified cre recombinase and LOX recombination sequences for inducible tissue-specific amplification of gene expression. Kaczmarczyk et al (Nucleic Acids Res 2001 Jun 15;29(12):E56-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8388" in your Materials and Methods section.