pSLQ1869-1 pHR: U6-SpsgSV40 CMV-mCherry
PurposeExpresses Sp sgSV40 gRNA with a mCherry fluorescent marker
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||84249||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Total vector size (bp) 7654
Vector typeMammalian Expression, Lentiviral, CRISPR
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameSp sgSV40
SpeciesSynthetic; S. pyogenes
- Promoter mouse U6
- Cloning method Restriction Enzyme
- 5′ cloning site BstXI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer GAGATCCAGTTTGGTTAGTACCGGG
- 3′ sequencing primer ATGCATGGCGGTAATACGGTTAT (Common Sequencing Primers)
Contains a CMV-mCherry fluorescent marker 3' of the sgRNA.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSLQ1869-1 pHR: U6-SpsgSV40 CMV-mCherry was a gift from Stanley Qi (Addgene plasmid # 84249 ; http://n2t.net/addgene:84249 ; RRID:Addgene_84249)
For your References section:Complex transcriptional modulation with orthogonal and inducible dCas9 regulators. Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS. Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042. 10.1038/nmeth.4042 PubMed 27776111