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Complex transcriptional modulation with orthogonal and inducible dCas9 regulators.
Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
PubMed Journal

Plasmids from Article

ID Plasmid  
84239pSLQ2817 pPB: CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9
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84240pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9
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84241pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9
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84242pSLQ2813 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-ABI-tagBFP-SpdCas9
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84243pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeocin-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9
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84244pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeocin-WPRE PGK-GAI-tagBFP-SadCas9
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84245pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9
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84246pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9
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84247pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9
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84248pSLQ1870-1 pHR: U6-SpsgTRE3G CMV-mCherry
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84249pSLQ1869-1 pHR: U6-SpsgSV40 CMV-mCherry
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84250pSLQ2806-2 pHR: U6-Sasgv2TRE3G CMV-mCherry
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84251pSLQ1852-2 pHR: U6-SpsgCD95-1 CMV-EGFP
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84252pSLQ1852-3 pHR: U6-SpsgCD95-2 CMV-EGFP
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84253pSLQ1852-5 pHR: U6-SpsgCD95-3 CMV-EGFP
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84254pSLQ2853-3 pHR: U6-Sasgv2CXCR4-1 CMV-EGFP
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84255pSLQ2853-4 pHR: U6-Sasgv2CXCR4-2 CMV-EGFP
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84256pSLQ2853-5 pHR: U6-Sasgv2CXCR4-3 CMV-EGFP
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84257pSLQ2821 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI
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84258pSLQ2804 pHR: U6-SpsgTRE3G CMV-PYL1-VPR-IRES-mCherry
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84260pSLQ2827 pHR: U6-SpsgSV40 CMV-PYL1-KRAB-IRES-mCherry
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84261pSLQ2816 pPB: CAG-PYL1-VPR-p2A-GID1-ABI-WPRE PGK-GAI-tagBFP-SpdCas9
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106895pSLQ2822 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI
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