PurposeChicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||92359||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3017
- Total vector size (bp) 3494
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namechick U6.3 promoter and gRNA cloning cassette
SpeciesG. gallus (chicken)
Insert Size (bp)477
- Promoter chick U6.3
- Cloning method Gibson Cloning
- 5′ sequencing primer gatcaagcctgattgggagaa (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bychick U6.3 promoter (Kudo and Sutou, 2005) was cloned into BsaI sites in pNG3 vector #30987
Terms and Licenses
Kudo, T., and S. Sutou. 2005. Usage of putative chicken U6 promoters for vector-based RNA interference. J Reprod Dev. 51:411-417.
Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Apr 14. ():. 10.1093/nar/gkr218 PubMed 21493687
Please visit https://www.biorxiv.org/content/early/2017/05/08/135525 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcU6_3 sgRNA was a gift from Tatjana Sauka-Spengler (Addgene plasmid # 92359 ; http://n2t.net/addgene:92359 ; RRID:Addgene_92359)
For your References section:Genome and epigenome engineering CRISPR toolkit for in vivo modulation of cis-regulatory interactions and gene expression in the chicken embryo. Williams RM, Senanayake U, Artibani M, Taylor G, Wells D, Ahmed AA, Sauka-Spengler T. Development. 2018 Feb 23;145(4). pii: dev.160333. doi: 10.1242/dev.160333. 10.1242/dev.160333 PubMed 29386245