Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Genome and epigenome engineering CRISPR toolkit for in vivo modulation of cis-regulatory interactions and gene expression in the chicken embryo.
Williams RM, Senanayake U, Artibani M, Taylor G, Wells D, Ahmed AA, Sauka-Spengler T
Development. 2018 Feb 23;145(4). pii: dev.160333. doi: 10.1242/dev.160333.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
92357pCAG MCP-VP64CAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.
Loading...
92358pCAG Cas9-2A-CitrineCAG-driven ubiquitous expression of Cas9. Contains 2A-Citrine reporter. For CRISPR mediated gene knockouts in chicken embryos.
Loading...
92359pcU6_3 sgRNAChicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter.
Loading...
92361pX330a dCas9-KRABModified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. For targeted enhancer inactivation in chicken embryos.
Loading...
92362pX330a dCas9-LSD1Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to LSD1. For targeted enhancer demethylation in chicken embryos.
Loading...
92363pX330a dCas9-VP64Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to VP64 transcriptional activation domain. For targeted gene activation in chicken embryos.
Loading...
92393pTK Cas9-2A-CitrineEnhancer/reporter plasmid for tissue-specific expression of Cas9 with 2A-Citrine reporter. Contains BsmBI-flanked LacZ cloning cassette for rapid GoldenGate-based cloning of specific enhancers.
Loading...
92394pcU6_3 MS2 sgRNAChick-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter, with tracrRNA scaffold containing stem loops allowing binding of bacteriophage MS2 coat protein MCP
Loading...
92395pcU6_1 sgRNAChicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_1 pol III promoter.
Loading...
92396pCAG dCas9-KRAB-2A-EGFPCAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.
Loading...
92397pCI Cas9 H2B-RFPCAG-driven ubiquitous expression of Cas9 with Histone2B-RFP reporter controlled by IRES
Loading...
92398pCI H2B-RFPUbiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-mRFP1 reporter.
Loading...
92399pCI H2B-GFPUbiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-EGFP reporter.
Loading...

Antibodies from Article