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Induction of targeted, heritable mutations in barley and Brassica oleracea using RNA-guided Cas9 nuclease.
Lawrenson T, Shorinola O, Stacey N, Li C, Ostergaard L, Patron N, Uauy C, Harwood W
Genome Biol. 2015 Nov 30;16(1):258. doi: 10.1186/s13059-015-0826-7.
PubMed

Plasmids from Article

ID Plasmid Purpose  
68252pICSL11055Level 1 Golden Gate Cassette: Plant kanamycin resistance cassette
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68253pICSL11057Level 1 Golden Gate Cassette: sgRNA cassette targeting PM19_1 in barley
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68254pICSL11058Level 1 Golden Gate Cassette: sgRNA cassette targeting PM19_3 in barley
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68255pICSL11061Level 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-1 in Brassica oleracea
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68256pICSL11062Level 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-2 in Brassica oleracea
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68257pICSL12009Level 0 Golden Gate Part: Promoter and 5UTR Ubiquitin (Zea mays)
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68258pICSL11056Level 1 Golden Gate Cassette: Cas9 expression cassette for monocotyledonous plants
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68259pICSL80036Level 0 Golden Gate Part: Coding sequence, hygromycin phophotransferase II (Escherichia coli)
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68260pICSL80037Level 0 Golden Gate Part: Coding sequence, neomycin phophotransferase II (Escherichia coli)
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68261pICSL90002Level 0 Golden Gate Part: Promoter U6-26 (Arabidopsis thaliana)
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68262pICSL90003Level 0 Golden Gate Part: Promoter U6 (Triticum aestivum)
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68263pICSL11059Level 1 Golden Gate Cassette: Plant hygromycin (with intron) resistance cassette
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68264pICSL11060Level 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea)
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