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The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble proteins for degradation.
Xu Y, Anderson DE, Ye Y
Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
86857pVENUS-N1-Ubl4ATo express Ubl4A tagged with VENUS at C terminus.
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86858pcDNA5/FRT/TO-FNTA-FLAGTo express FNTA with a FLAG tag at C terminus.
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86859pVENUS-N1-FNTATo express FNTA with a Venus tag at C terminus.
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86869pcDNA5/FRT/TO-FNTB-HATo express FNTB with a HA tag at C terminus.
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86870pEGFP-C1-GFP-SF3B6-FLAGTo express SF3B6 with a GFP and FLAG tags at N- and C-terminus, respectively.
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86871pEGFP-C1-GFP-SF3B6-Delete-NLS-FLAGTo express SF3B6 delete NLS signal with a GFP and FLAG tags at N- and C-terminus, respectively.
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86872pVENUS-N1-POLR2G-GFPTo express POLR2G (RPB7) tagged with VENUS at C terminus.
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86873pcDNA5/FRT/TO-GRB2-GFPTo express GRB2 with a GFP tag at C terminus.
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86874pcDNA5/FRT/TO-FLAG-MORF4L1(iso2)To express MORF4L1 (iso2) with a FLAG tag at N-terminus.
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86875pcDNA5/FRT/TO-FLAG-CHK1To express CHK1 with a FLAG tag at N-terminus.
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86921pEGFP-C1-FLAG-GFP-Ubl4ATo express Ubl4A tagged with FLAG-EGFP at N- terminus.
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86922pEGFP-C1-FLAG-GFP-Ubl4A-N(1-89)To express Ubl4A (1-89 residues) tagged with FLAG-EGFP at N- terminus.
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86923pEGFP-C1-FLAG-GFP-Ubl4A-C(90-157)To express Ubl4A (90-157 residues) tagged with FLAG-EGFP at N- terminus.
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86924HUWE1-sgRNA-1To express sgRNA target HUWE1 gene
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86925HUWE1-sgRNA-2To express sgRNA target HUWE1 gene
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87202pEGFP-C1-FLAG-GFP-Ubl4A-H1(90-107)To express Ubl4A (90-107 residues) tagged with FLAG-EGFP at N- terminus.
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87203pEGFP-C1-FLAG-GFP-Ubl4A-H2(108-130)To express Ubl4A (108-130 residues) tagged with FLAG-EGFP at N- terminus.
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