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The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble proteins for degradation.
Xu Y, Anderson DE, Ye Y
Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.
PubMed Article

Plasmids from Article

ID Plasmid  
86857pVENUS-N1-Ubl4A
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86858pcDNA5/FRT/TO-FNTA-FLAG
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86859pVENUS-N1-FNTA
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86869pcDNA5/FRT/TO-FNTB-HA
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86870pEGFP-C1-GFP-SF3B6-FLAG
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86871pEGFP-C1-GFP-SF3B6-Delete-NLS-FLAG
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86872pVENUS-N1-POLR2G-GFP
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86873pcDNA5/FRT/TO-GRB2-GFP
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86874pcDNA5/FRT/TO-FLAG-MORF4L1(iso2)
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86875pcDNA5/FRT/TO-FLAG-CHK1
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86921pEGFP-C1-FLAG-GFP-Ubl4A
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86922pEGFP-C1-FLAG-GFP-Ubl4A-N(1-89)
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86923pEGFP-C1-FLAG-GFP-Ubl4A-C(90-157)
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86924HUWE1-sgRNA-1
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86925HUWE1-sgRNA-2
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87202pEGFP-C1-FLAG-GFP-Ubl4A-H1(90-107)
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87203pEGFP-C1-FLAG-GFP-Ubl4A-H2(108-130)
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