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Jonathan Ting Lab: ChIEF and oChIEF AAV vectors
Unpublished

Plasmids from Article

ID Plasmid Purpose
51092pAAV-CaMKIIa-oChIEF(E163A/T199C)-P2A-mKate2Forebrain principal neuron expression of oChIEF kinetic variant E163A/T199C with physically uncoupled mKate2 red fluorophore
51093pAAV-CaMKIIa-oChIEF(E163A/T199C)-P2A-EGFPForebrain principal neuron expression of oChIEF kinetic variant E163A/T199C with physically uncoupled EGFP fluorophore
51094AAV-EF1a-DIO-oChIEF(E163A/T199C)-P2A-dTomato-WPRE-BGHpACre dependent expression of oChIEF kinetic variant E163A/T199C with physically uncoupled dTomato fluorophore
51095AAV-EF1a-DIO-ChIEF(E162A/T198C)-P2A-dTomato-WPRE-BGHpACre dependent expression of ChIEF kinetic variant E162A/T198C with physically uncoupled dTomato fluorophore

Antibodies from Article