Addgene - pCS-CG Plasmid Data

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Inder Verma Lab Plasmids

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Plasmid 12154: pCS-CG

Gene/insert name:		CMV-GFP
Alternative names:		CMV GFP
		GFP
Insert size (bp):		1376
Species of gene(s):		Other
Vector backbone:		pCS (Search Vector Database)
Backbone manufacturer:		Verma Lab
Type of vector:		Mammalian expression,Lentiviral
Backbone size (bp):		7088
Cloning site 5':		BamHI
Site destroyed during cloning:		No
Cloning site 3':		XhoI
Site destroyed during cloning:		No
5' Sequencing primer:		n/a (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		Low Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Plasmid Provided In:		Stbl3
Principal Investigator:		Inder M Verma
Terms and Licenses:		MTA

Comments: pCS has a 133-bp deletion in the 3' LTR, generating a self-inactivating (SIN) virus. See article for cloning details.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Selected features

CAG_enhancer

315 - 602

CMV_immearly_promoter

236 - 812

CMV_fwd_primer

769 - 789

HIV-1_5_LTR

832 - 1012

truncHIV-1_3_LTR

832 - 1012

HIV-1_psi_pack

1120 - 1164

RRE

1674 - 1907

ORF frame 1

1552 - 2409

CAG_enhancer

2464 - 2751

CMV_immearly_promoter

2409 - 2961

CMV_fwd_primer

2918 - 2938

CMV_promoter

2919 - 2988

EGFP_N_primer

3078 - 3057

EGFP

3012 - 3728

ORF frame 3

3012 - 3749

EGFP_C_primer

3665 - 3686

cPPT

3928 - 3943

U3PPT

3928 - 3949

HIV-1_5_LTR

4265 - 4445

truncHIV-1_3_LTR

4265 - 4445

BGH_rev_primer

4488 - 4471

bGH_PA_terminator

4474 - 4701

f1_origin

4764 - 5070

pBABE_3_primer

5204 - 5184

SV40_enhancer

5406 - 5190

SV40_promoter

5202 - 5471

SV40_origin

5370 - 5447

SV40pro_F_primer

5432 - 5451

EM7_promoter

5565 - 5632

bleo

5633 - 6004

sh_ble

5633 - 6007

SV40_PA_terminator

6140 - 6259

EBV_rev_primer

6228 - 6247

M13_reverse_primer

6321 - 6303

M13_pUC_rev_primer

6342 - 6320

lac_promoter

6385 - 6356

pBR322_origin

7313 - 6694

ORF frame 1

8328 - 7468

Ampicillin

8328 - 7468

AmpR_promoter

8398 - 8370

Unique restriction sites

NarI		1016
NotI		1519
BamHI		2380
PstI		2396
EcoRI		2398
NheI		2990
AgeI		2999
XhoI		3756
KpnI		3878
ApaI		4450
StuI		5503
MscI		5636
FspI		7763

Article: Development of a self-inactivating lentivirus vector. Miyoshi H et al. (J Virol. 1998 Oct . 72(10):8150-7. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 12154" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.