Showing: 21 - 40 of 64 results
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Plasmid#59855PurposeEncodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.DepositorInsertCRISPR sg290860-6
UseCRISPR; ; toxoplasma gondiiTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTUB1:OsTIR1-3FLAG, SAG1:CAT
Plasmid#87258PurposeTIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.DepositorInsertsTIR1
CAT
UseToxoplasma expressionTags3FLAGExpressionMutationCodon optimized for Toxoplasma gondii expressionPromoterTgSAG1 361 bp and TgTUB1 2717 bpAvailabilityAcademic Institutions and Nonprofits only -
pTUB1:YFP-mAID-3HA, DHFR-TS:HXGPRT
Plasmid#87259PurposeYFP-mAID-3HA fusion driven by a minimal TUB1 promoter with an HXGdrug selectable marker. The mAID CDS is from Arabidopsis thaliana auxin-responsive protein IAA17E66-S133DepositorInsertsYFP-mAID-3HA
HXGPRT
UseToxoplasma expressionTags3HA and mAIDExpressionMutationCodon optimized for Toxoplasma gondii expressionPromoterTgDHFR-TS 463 bp and TgTUB1 378 bpAvailabilityAcademic Institutions and Nonprofits only -
pTet-On-TAP
Plasmid#59017PurposeConstruct allows tetracycline inducible expression of a protein of interest fused to a tandem affinity purification tag (TAP) in toxoplasma gondii.DepositorTypeEmpty backboneUseToxoplasma expressionTagsCalmodulin Binding Peptide fused with a TEV Cleav…ExpressionMutationPromoterRPS13 with Tet Operators InsertedAvailabilityAcademic Institutions and Nonprofits only -
pFloxed DHFR-TS*
Plasmid#100605PurposepUC19 with loxP-pDHFR-TS:DHFR-TS*(Pyr-R)-Stop-loxP-DHFR-TS 3' UTRDepositorInsertFloxed TgDHFR-TS*
UseToxoplasma expressionTagsExpressionMutationPyrimethamine resistancePromoterAvailabilityAcademic Institutions and Nonprofits only -
pLinker-2xTy-HXGPRT-LoxP
Plasmid#86664PurposeCRISPR tagging with 2xTy in Toxoplamsa gondiiDepositorInsertLinker-2xTy-HXGPRT 3'UTR
UseExpression in toxoplasma gondiiTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pUPRT::Floxed DHFR-TS*
Plasmid#100606PurposeTgUPRT disruption by knock in with Floxed DHFR-TS*(Pyr-R) cassette with UPRT homology flanks.DepositorInsertTgUPRT::Floxed TgDHFR-TS*
UseToxoplasma expressionTagsExpressionMutationPyrimethamine resistancePromoterpTgDHFR-TSAvailabilityAcademic Institutions and Nonprofits only -
MIC2
Plasmid#50050Purposeexpresses toxoplasma gondii MIC2 extracellular domain in mammalian cellsDepositorInsertMIC2
UseTagsHIS, SBP, and TEVExpressionMammalianMutationS158A, N357S, N463S, N470DPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
m2ap
Plasmid#50051Purposeexpresses toxoplasma gondii MIC2 associated protein (m2ap) in mammalian cellsDepositorInsertM2AP
UseTagsHISExpressionMammalianMutationstarts at aa47; N127R, S158A, S177N, N357S, N463S…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pmCherry.LIC.HXGPRT
Plasmid#83364PurposePlasmid contains LIC cassette upstream of an in-frame copy of mCherry and HXGPRT selectable marker cassette for endogenous tagging on the gene locusDepositorInsertmCherry
UseToxoplasma gondiiTagsmCherryExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
ptdTomato.LIC.HXGPRT
Plasmid#83365PurposePlasmid contains LIC cassette upstream of an in-frame copy of tdTomato and HXGPRT selectable marker cassette for endogenous tagging on the gene locusDepositorInserttdTomato
UseToxoplasma gondiiTagstdTomatoExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCFP.HXGPRT
Plasmid#83366PurposePlasmid contains LIC cassette upstream of an in-frame copy of CFP and HXGPRT selectable marker cassette for endogenous tagging on the gene locusDepositorInsertCFP
UseToxoplasma gondiiTagsCFPExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSF-TAP.LIC.HXGPRT
Plasmid#83367PurposePlasmid contains LIC cassette upstream of an in-frame copy of SF-TAP and HXGPRT selectable marker cassette for endogenous tagging on the gene locusDepositorInsertStrep-FLAG
UseToxoplasma gondiiTagsSF-TAPExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pYFP.LIC.DHFR
Plasmid#83114PurposePlasmid contains LIC cassette upstream of an in-frame copy of YFP and DHFR-TS selectable marker cassette for endogenous tagging on the gene locus.DepositorInsertYFP
UseToxoplasma gondiiTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pYFP.LIC.HXGPRT
Plasmid#83128PurposePlasmid contains LIC cassette upstream of an in-frame copy of YFP and HXGPRT selectable marker cassette for endogenous tagging on the gene locus.DepositorInsertYFP
UseToxoplasma gondiiTagsYFPExpressionMutationPromoterEndogenous GeneAvailabilityAcademic Institutions and Nonprofits only -
pTUB1:YFP-AID-3HA, DHFR-TS:HXGPRT
Plasmid#87260PurposeYFP-AID-3HA fusion driven by a minimal TUB1 promoter with an HXGPRT drug selectable marker. The AID CDS is Arabidopsis thaliana auxin-responsive protein IAA17.DepositorInsertsYFP-AID-3HA
HXGPRT
UseToxoplasma expressionTags3HA and AIDExpressionMutationPromoterTgDHFR-TS 463 bp and TgTUB1 378 bpAvailabilityAcademic Institutions and Nonprofits only -
pU6-SAG1-DHFR
Plasmid#80322PurposeEncodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamineDepositorInsertSAG1 sgRNA
UseCRISPRTagsExpressionMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pU6-Decoy
Plasmid#80324PurposeEncodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondiiDepositorInsertDecoy sgRNA
UseCRISPRTagsExpressionMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
ATSR
Plasmid#50052Purposeexpresses t.gondii VWA+TSR1 domains in mammalian cellsDepositorInsertMIC2-ATSR
UseTagsHISExpressionMammalianMutationS158APromoterChick beta-actin promoterAvailabilityAcademic Institutions and Nonprofits only -
proATSR
Plasmid#50053PurposeExpresses T.gondii prodomain+VWA+TSR1 domains in mammalian cellsDepositorInsertMIC2-proATSR
UseTagsHISExpressionMammalianMutationS158APromoterchick beta-actin promoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 21 - 40 of 64 results