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Addgene
Showing: 281 - 300 of 2339 results

  1. pGL4.23 C5_12

    Plasmid
    #60319
    Purpose
    This plasmid contains a genomic fragment that is not active in human pancreatic islets, a minimal promoter and a luc2 gene. Can be used as negative control in reporter assays performed in human pancreatic islets.
    Depositor
    Jorge Ferrer
    Article
    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1
    Insert
    Non active element (nearest TSS C18orf26)
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  2. pGL4.23 C5_14

    Plasmid
    #60321
    Purpose
    This plasmid contains a genomic fragment that is not active in human pancreatic islets, a minimal promoter and a luc2 gene. Can be used as negative control in reporter assays performed in human pancreatic islets.
    Depositor
    Jorge Ferrer
    Article
    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1
    Insert
    Non active element (nearest TSS CCRN4L)
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  3. pGL4.23 C5_15

    Plasmid
    #60322
    Purpose
    This plasmid contains a genomic fragment that is not active in human pancreatic islets, a minimal promoter and a luc2 gene. Can be used as negative control in reporter assays performed in human pancreatic islets.
    Depositor
    Jorge Ferrer
    Article
    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1
    Insert
    Non active element (nearest TSS PHLDA1) (PHLDA1 Human)
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  4. pGL4.23-GW

    Plasmid
    #60323
    Purpose
    This is a modified version of the pGL4.23 vector from Promega, containing a Gateway cassette upstream of the minimal promoter. This vector can be used for for Gateway cloning of candidate enhancer elements. As negative control, use pGL4.23 without the gateway cassette (available from Promega).
    Depositor
    Jorge Ferrer
    Article
    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    60323-plasmid-map-sequence-id-316810

    Enlarge

  6. pGL2 HNF4A P2 mut

    Plasmid
    #60325
    Purpose
    Mutated HNF4A P2 promoter region cloned into the pGL2-basic backbone. The -181 G to A mutation is in a HNF1alpha binding site and cosegregates with MODY.
    Depositor
    Jorge Ferrer
    Article
    Hansen et al J Clin Invest. 2002 Sep;110(6):827-33.
    Insert
    HNF4A P2 mut (-181 G to A) (HNF4A Human)
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    The -181 G to A mutation is in a HNF1alpha bindin…
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  7. pSynSRE-T-Luc

    Plasmid
    #60444
    Purpose
    Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.
    Depositor
    Timothy Osborne
    Article
    Dooley et al J Biol Chem. 1998 Jan 16;273(3):1349-56.
    Insert
    HMG-CoA synthase promoter (-324/-225)
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    HMG-CoA synthase (-324/-225) & TATAA (-28/+39)
    Availability
    Academic Institutions and Nonprofits only
    60444-plasmid-map-sequence-id-266122

    Enlarge

  8. pSynSRE-Mut-T-Luc

    Plasmid
    #60490
    Purpose
    Contains a mutant version of the WT pSynSRE-T-Luc reporter plasmid. Four point mutations in the promoter SRE elements abolish SREBP-induced luciferase expression as described by Smith et al., 1988
    Depositor
    Timothy Osborne
    Article
    Smith et al J Biol Chem. 1988 Dec 5;263(34):18480-7.
    Insert
    HMG-CoA synthase promoter (-324/-225) fused to HMG-CoA synthase TATAA (-28/+39) transcription initiator sequence
    Use
    Luciferase
    Tags
    Luciferase reporter
    Expression
    Mammalian
    Mutation
    Four point mutations (G->C or A->C) in the …
    Promoter
    Partial HMG-CoA synthase promoter (-324/-225) fus…
    Availability
    Academic Institutions and Nonprofits only
    60490-plasmid-map-sequence-id-332535

    Enlarge

  10. pIS1 ATP2B1

    Plasmid
    #60788
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains ATP2B1 3' UTR and wild-type miR-155 sites
    Depositor
    David Bartel
    Article
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Insert
    ATP2B1 3'UTR and wild-type miR-155 binding site (ATP2B1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    thymidine kinase (HSV-TK promoter)
    Availability
    Academic Institutions and Nonprofits only
    60788-plasmid-map-sequence-id-97788

    Enlarge

  11. pIS1 mATP2B1

    Plasmid
    #60789
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains ATP2B1 3' UTR and mutated miR-155 sites
    Depositor
    David Bartel
    Article
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Insert
    ATP2B1 3'UTR and mutated miR-155 binding site (ATP2B1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    mutated miR-155 binding site
    Promoter
    thymidine kinase (HSV-TK promoter)
    Availability
    Academic Institutions and Nonprofits only
    60789-plasmid-map-sequence-id-97792

    Enlarge

  12. pIS1 ELOVL6

    Plasmid
    #60790
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains ELOVL6 3' UTR and wild-type miR-155 sites
    Depositor
    David Bartel
    Article
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Insert
    ELOVL6 3'UTR and wild-type miR-155 binding site (ELOVL6 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    thymidine kinase (HSV-TK promoter)
    Availability
    Academic Institutions and Nonprofits only
    60790-plasmid-map-sequence-id-97799

    Enlarge

  13. pIS1 mELOVL6

    Plasmid
    #60791
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains ELOVL6 3' UTR and mutated miR-155 sites
    Depositor
    David Bartel
    Article
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Insert
    ELOVL6 3'UTR and mutated miR-155 binding site (ELOVL6 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    mutated miR-155 binding site
    Promoter
    thymidine kinase (HSV-TK promoter)
    Availability
    Academic Institutions and Nonprofits only
    60791-plasmid-map-sequence-id-112566

    Enlarge

  14. pIS1 EPAS1

    Plasmid
    #60792
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains EPAS1 3' UTR and wild-type miR-155 sites
    Depositor
    David Bartel
    Article
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Insert
    EPAS1 3'UTR and wild-type miR-155 binding site (EPAS1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    thymidine kinase (HSV-TK promoter)
    Availability
    Academic Institutions and Nonprofits only
    60792-plasmid-map-sequence-id-97802

    Enlarge

  15. pIS1 mEPAS1

    Plasmid
    #60793
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains EPAS1 3' UTR and mutated miR-155 sites
    Depositor
    David Bartel
    Article
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Insert
    EPAS1 3'UTR and mutated miR-155 binding site (EPAS1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    mutated miR-155 binding site
    Promoter
    thymidine kinase (HSV-TK promoter)
    Availability
    Academic Institutions and Nonprofits only
    60793-plasmid-map-sequence-id-112560

    Enlarge

  16. pIS1 HK1

    Plasmid
    #60794
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains HK1 3' UTR and wild-type miR-155 sites
    Depositor
    David Bartel
    Article
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Insert
    HK1 3'UTR and wild-type miR-155 binding site (HK1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    thymidine kinase (HSV-TK promoter)
    Availability
    Academic Institutions and Nonprofits only
    60794-plasmid-map-sequence-id-97804

    Enlarge

  17. pIS1 mHK1

    Plasmid
    #60795
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains HK1 3' UTR and mutated miR-155 sites
    Depositor
    David Bartel
    Article
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Insert
    HK1 3'UTR and mutated miR-155 binding site (HK1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    mutated miR-155 binding site
    Promoter
    thymidine kinase (HSV-TK promoter)
    Availability
    Academic Institutions and Nonprofits only
    60795-plasmid-map-sequence-id-97806

    Enlarge

  18. pIS1 IL6R

    Plasmid
    #60796
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains IL6R 3' UTR and wild-type miR-155 sites
    Depositor
    David Bartel
    Article
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Insert
    IL6R 3'UTR and wild-type miR-155 binding site (IL6R Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    thymidine kinase (HSV-TK promoter)
    Availability
    Academic Institutions and Nonprofits only
    60796-plasmid-map-sequence-id-97807

    Enlarge

  19. pIS1 mIL6R

    Plasmid
    #60797
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains IL6R 3' UTR and mutated miR-155 sites
    Depositor
    David Bartel
    Article
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Insert
    IL6R 3'UTR and mutated miR-155 binding site (IL6R Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    mutated miR-155 binding site
    Promoter
    thymidine kinase (HSV-TK promoter)
    Availability
    Academic Institutions and Nonprofits only
    60797-plasmid-map-sequence-id-97808

    Enlarge

  20. pIS1 INSIG2

    Plasmid
    #60798
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains INSIG2 3' UTR and wild-type miR-155 sites
    Depositor
    David Bartel
    Article
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Insert
    INSIG2 3'UTR and wild-type miR-155 binding site (INSIG2 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    thymidine kinase (HSV-TK promoter)
    Availability
    Academic Institutions and Nonprofits only
    60798-plasmid-map-sequence-id-97809

    Enlarge

Showing: 281 - 300 of 2339 results