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Showing: 21 - 40 of 174 results

  1. 6NIM

    Plasmid
    #127294
    Purpose
    Bacterial expression for structure determination. May not contain entire coding region of gene
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-BRD18
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    127294-plasmid-map-sequence-id-247944

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  4. pSMOX-mNG::LacI-V3

    Plasmid
    #210224
    Purpose
    Integrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.
    Depositor
    Calvin Tiengwe
    Article
    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115
    Insert
    mNeongreen Lac repressor fusion protein
    Use
    Trypanosoma brucei expression
    Tags
    Expression
    Mutation
    Promoter
    Pol II readthrough transcription after integration
    Availability
    Academic Institutions and Nonprofits only
    210224-plasmid-map-sequence-id-415797

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  5. PP-PMM

    Plasmid
    #25202
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Tb10.70.0370:M1-K245
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids M1-K245.
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  6. PP-UDPGP

    Plasmid
    #25285
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Tb10.389.0330:L3-E485
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids L3-E485
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  7. 5KO4 (PP-BRD19)

    Plasmid
    #101882
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-BRD19
    Use
    Tags
    6xHis, TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    101882-plasmid-map-sequence-id-194517

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  8. PP-HSP90 (3O6O)

    Plasmid
    #28153
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HSP90
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    N-terminal domain
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  9. pTrex-n-eGFP

    Plasmid
    #62544
    Purpose
    Expression of eGFP in T. cruzi. The NheI-linearized form of this plasmid could be efficiently integrated in to T. cruzi genome
    Depositor
    Rick Tarleton
    Article
    Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.
    Insert
    eGFP
    Use
    (1)trypanosoma cruzi expression of egfp (2) genom…
    Tags
    None
    Expression
    Mutation
    None
    Promoter
    Ribo-HX1
    Availability
    Academic Institutions and Nonprofits only
    62544-plasmid-map-sequence-id-109759

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  10. 5K29 (PP-BRD3)

    Plasmid
    #101883
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-BRD3
    Use
    Tags
    6xHis, TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    101883-plasmid-map-sequence-id-194713

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  20. Cas9/pTREX-n

    Plasmid
    #68708
    Purpose
    Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Insert
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
    Use
    CRISPR
    Tags
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …
    Expression
    Mutation
    C4239T mutation that eliminates a BamHI restricti…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    68708-plasmid-map-sequence-id-123989

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Showing: 21 - 40 of 174 results