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XanthoMoClo Xanthobacter Genetic Toolkit
(Kit # 1000000259 )

Depositing Lab:   Michael Springer

The XanthoMoClo collection contains a set of 160 standardized and characterized plasmids and parts used for the modular assembly of single and multi-gene plasmids for any Xanthobacter or Roseixanthobacter strain. Also included in the kit is the conjugation donor strain Escherichia coli DH10B-MOB (Addgene #229395) that can deliver plasmids to recipient cells (i.e. to transform Xanthobacter).

This kit will be sent as bacterial glycerol stocks in 96-well plate format.

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$704 USD + shipping
Available to academics and nonprofits only.
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Original Publication

XanthoMoClo—A Robust Modular Cloning Genetic Toolkit for the Genera Xanthobacter and Roseixanthobacter. Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, Springer M. ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 10.1021/acssynbio.4c00806. PubMed (Link opens in a new window) Article (Link opens in a new window).

Description

The XanthoMoClo collection contains a set of 160 standardized and characterized plasmids and parts used for the modular assembly of single and multi-gene plasmids for any Xanthobacter and Roseixanthobacter strain. The kit employs a modular cloning strategy with Golden Gate plasmid assembly using BsaI or BsmBI enzymes, though the overhang sequences may not be compatible with all standard MoClo parts.

The kit includes 152 plasmid parts such as promoters, terminators, origins of replication, antibiotic selection markers, fluorescent proteins, and an origin of transfer (for conjugation). Also included are an entry vector for incorporating your own additional parts into the kit and seven pre-assembled backbones ready for your own gene expression cassette.

In addition to the plasmids, the kit includes the conjugation donor strain E. coli DH10B-MOB (Addgene #229395) that can deliver plasmids with an origin of transfer into recipient cells (i.e. to transform Xanthobacter).

Schematic describing the types of plasmid parts included in the kit: "Standardized Parts & Backbones", "Multi & Single Copy Origins", "Constitutive & Inducible Promoters", "Fluorescent Proteins & Antibiotic Markers", and "Conjugation Donor for Easy Transformation".

Figure 1: Overview of the XanthoMoClo Xanthobacter Genetic toolkit.

Kit Documentation

For protocols and detailed guidance on using the toolkit, please refer to the supplemental materials from Soltysiak and Ory et al., 2025 (Link opens in a new window).

XanthoMoClo Kit Plasmid Maps (ZIP, 386 KB)

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“The XanthoMoClo Xanthobacter Genetic Toolkit was a gift from Michael Springer (Addgene kit #1000000259).”

For your Reference section:

XanthoMoClo—A Robust Modular Cloning Genetic Toolkit for the Genera Xanthobacter and Roseixanthobacter. Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, Springer M. ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 10.1021/acssynbio.4c00806. PubMed (Link opens in a new window) Article (Link opens in a new window).

XanthoMoClo Xanthobacter Genetic Toolkit - #1000000259

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right, as well as lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

 Ampicillin
Kanamycin
60 ug/mL of DAP

Inventory

Well Plasmid Resistance
Plate 1 / A / 1 pXTK000 [Marker_KanR-NeoR-neo_PartType2]
Ampicillin
Plate 1 / A / 2 pXTK001 [Marker_KanR-NeoR-nptII_PartType2]
Ampicillin
Plate 1 / A / 3 pXTK002 [Marker_TetR-tetAR_PartType2]
Ampicillin
Plate 1 / A / 4 pXTK003 [Marker_GenR-aacC1_PartType2]
Ampicillin
Plate 1 / A / 5 pXTK004 [Marker_SpcR-StrR-aadA_PartType2]
Ampicillin
Plate 1 / A / 6 pXTK005 [Marker_AmpR-bla_PartType2]
Ampicillin
Plate 1 / A / 7 pXTK006 [Marker_CamR-cat_PartType2]
Ampicillin
Plate 1 / A / 8 pXTK007 [Marker_KanR-NeoR-neo_PartType4]
Ampicillin
Plate 1 / A / 9 pXTK008 [Marker_KanR-NeoR-nptII_PartType4]
Ampicillin
Plate 1 / A / 10 pXTK009 [Marker_TetR-tetAR_PartType4]
Ampicillin
Plate 1 / A / 11 pXTK010 [Marker_GenR-aacC1_PartType4]
Ampicillin
Plate 1 / A / 12 pXTK011 [Marker_SpcR-StrR-aadA_PartType4]
Ampicillin
Plate 1 / B / 1 pXTK012 [Marker_AmpR-bla_PartType4]
Ampicillin
Plate 1 / B / 2 pXTK013 [Marker_CamR-cat_PartType4]
Ampicillin
Plate 1 / B / 3 pXTK014 [Marker_SacB_PartType4]
Ampicillin
Plate 1 / B / 4 pXTK015 [Marker_SacB_PartType5-7]
Ampicillin
Plate 1 / B / 5 pXTK016 [Marker_SacB_PartType8-10]
Ampicillin
Plate 1 / B / 6 pXTK017 [Origin_pMB1_PartType1]
Ampicillin
Plate 1 / B / 7 pXTK018 [Origin_pUC_PartType1]
Ampicillin
Plate 1 / B / 8 pXTK019 [Origin_pBBR1_PartType1]
Ampicillin
Plate 1 / B / 9 pXTK020 [Origin_p15A_PartType1]
Ampicillin
Plate 1 / B / 10 pXTK021 [Origin_pBR322-ROP_PartType1]
Ampicillin
Plate 1 / B / 11 pXTK022 [Origin_pRO1600-ColE1_PartType1]
Ampicillin
Plate 1 / B / 12 pXTK023 [Origin_pSC101hs_PartType1]
Ampicillin
Plate 1 / C / 1 pXTK024 [Origin_RSF1010_PartType1]
Ampicillin
Plate 1 / C / 2 pXTK025 [Origin_RK2-oriT_PartType1]
Ampicillin
Plate 1 / C / 3 pXTK026 [Origin_repABC-pMlb_PartType3]
Ampicillin
Plate 1 / C / 4 pXTK027 [Origin_repABC-pMla_PartType3]
Ampicillin
Plate 1 / C / 5 pXTK028 [Origin_repABC-p42b_PartType3]
Ampicillin
Plate 1 / C / 6 pXTK029 [Origin_repABC-p42e_PartType3]
Ampicillin
Plate 1 / C / 7 pXTK030 [Origin_repABC-p42f1_PartType3]
Ampicillin
Plate 1 / C / 8 pXTK031 [Origin_repABC-p42f2_PartType3]
Ampicillin
Plate 1 / C / 9 pXTK032 [Origin_repABC-pTi_PartType3]
Ampicillin
Plate 1 / C / 10 pXTK033 [Origin_repABC-pSymB_PartType3]
Ampicillin
Plate 1 / C / 11 pXTK034 [Origin_repABC-pSymA_PartType3]
Ampicillin
Plate 1 / C / 12 pXTK035 [Origin_RK2-oriT_PartType3]
Ampicillin
Plate 1 / D / 1 pXTK036 [Con-Pro_rpsM-Xantho_PartType5]
Ampicillin
Plate 1 / D / 2 pXTK037 [Con-Pro_glpT-Ecoli_PartType5]
Ampicillin
Plate 1 / D / 3 pXTK038 [Con-Pro_rpsL-Xantho_PartType5]
Ampicillin
Plate 1 / D / 4 pXTK039 [Con-Pro_sacB-Bacillus_PartType5]
Ampicillin
Plate 1 / D / 5 pXTK040 [Con-Pro_cat-Broad_PartType5]
Ampicillin
Plate 1 / D / 6 pXTK041 [Con-Pro_aadA-Broad_PartType5]
Ampicillin
Plate 1 / D / 7 pXTK042 [Con-Pro_acc3-Broad_PartType5]
Ampicillin
Plate 1 / D / 8 pXTK043 [Con-Pro_bla-v1-Broad_PartType5]
Ampicillin
Plate 1 / D / 9 pXTK044 [Con-Pro_ipxC-Xantho_PartType5]
Ampicillin
Plate 1 / D / 10 pXTK045 [Con-Pro_bla-v2-Broad_PartType5]
Ampicillin
Plate 1 / D / 11 pXTK046 [Con-Pro_panD-Xantho_PartType5]
Ampicillin
Plate 1 / D / 12 pXTK047 [Con-Pro_nptII-Broad_PartType5]
Ampicillin
Plate 1 / E / 1 pXTK048 [Con-Pro_murB-Xantho_PartType5]
Ampicillin
Plate 1 / E / 2 pXTK049 [Con-Pro_aacC1-Broad_PartType5]
Ampicillin
Plate 1 / E / 3 pXTK050 [Con-Pro_neo-Broad_PartType5]
Ampicillin
Plate 1 / E / 4 pXTK051 [Con-Pro_tetA-v1-Broad_PartType5]
Ampicillin
Plate 1 / E / 5 pXTK052 [Con-Pro_tetA-v2-Broad_PartType5]
Ampicillin
Plate 1 / E / 6 pXTK053 [Con-Pro_UDP-Xantho_PartType5]
Ampicillin
Plate 1 / E / 7 pXTK054 [Ind-Pro_tetAR-Broad_PartType5]
Ampicillin
Plate 1 / E / 8 pXTK055 [Con-Pro_rpsM-Xantho_PartType8]
Ampicillin
Plate 1 / E / 9 pXTK056 [Con-Pro_glpT-Ecoli_PartType8]
Ampicillin
Plate 1 / E / 10 pXTK057 [Con-Pro_rpsL-Xantho_PartType8]
Ampicillin
Plate 1 / E / 11 pXTK058 [Con-Pro_sacB-Bacillus_PartType8]
Ampicillin
Plate 1 / E / 12 pXTK059 [Con-Pro_cat-Broad_PartType8]
Ampicillin
Plate 1 / F / 1 pXTK060 [Con-Pro_aadA-Broad_PartType8]
Ampicillin
Plate 1 / F / 2 pXTK061 [Con-Pro_acc3-Broad_PartType8]
Ampicillin
Plate 1 / F / 3 pXTK062 [Con-Pro_bla-v1-Broad_PartType8]
Ampicillin
Plate 1 / F / 4 pXTK063 [Con-Pro_ipxC-Xantho_PartType8]
Ampicillin
Plate 1 / F / 5 pXTK064 [Con-Pro_bla-v2-Broad_PartType8]
Ampicillin
Plate 1 / F / 6 pXTK065 [Con-Pro_panD-Xantho_PartType8]
Ampicillin
Plate 1 / F / 7 pXTK066 [Con-Pro_nptII-Broad_PartType8]
Ampicillin
Plate 1 / F / 8 pXTK067 [Con-Pro_murB-Xantho_PartType8]
Ampicillin
Plate 1 / F / 9 pXTK068 [Con-Pro_aacC1-Broad_PartType8]
Ampicillin
Plate 1 / F / 10 pXTK069 [Con-Pro_neo-Broad_PartType8]
Ampicillin
Plate 1 / F / 11 pXTK070 [Con-Pro_tetA-v1-Broad_PartType8]
Ampicillin
Plate 1 / F / 12 pXTK071 [Con-Pro_tetA-v2-Broad_PartType8]
Ampicillin
Plate 1 / G / 1 pXTK072 [Con-Pro_UDP-Xantho_PartType8]
Ampicillin
Plate 1 / G / 2 pXTK073 [Ind-Pro_tetAR-Broad_PartType8]
Ampicillin
Plate 1 / G / 3 pXTK074 [CDS_mRFP_PartType6]
Ampicillin
Plate 1 / G / 4 pXTK075 [CDS_sfGFP_PartType6]
Ampicillin
Plate 1 / G / 5 pXTK076 [CDS_CFP_PartType6]
Ampicillin
Plate 1 / G / 6 pXTK077 [CDS_EBFP2_PartType6]
Ampicillin
Plate 1 / G / 7 pXTK078 [CDS_GFP_PartType6]
Ampicillin
Plate 1 / G / 8 pXTK079 [CDS_mBlueberry2_PartType6]
Ampicillin
Plate 1 / G / 9 pXTK080 [CDS_mCerulean_PartType6]
Ampicillin
Plate 1 / G / 10 pXTK081 [CDS_mKalama1_PartType6]
Ampicillin
Plate 1 / G / 11 pXTK082 [CDS_mTurquoise_PartType6]
Ampicillin
Plate 1 / G / 12 pXTK083 [CDS_YFP_PartType6]
Ampicillin
Plate 1 / H / 1 pXTK084 [CDS_mRFP_PartType9]
Ampicillin
Plate 1 / H / 2 pXTK085 [CDS_sfGFP_PartType9]
Ampicillin
Plate 1 / H / 3 pXTK086 [CDS_CFP_PartType9]
Ampicillin
Plate 1 / H / 4 pXTK087 [CDS_EBFP2_PartType9]
Ampicillin
Plate 1 / H / 5 pXTK088 [CDS_GFP_PartType9]
Ampicillin
Plate 1 / H / 6 pXTK089 [CDS_mBlueberry2_PartType9]
Ampicillin
Plate 1 / H / 7 pXTK090 [CDS_mCerulean_PartType9]
Ampicillin
Plate 1 / H / 8 pXTK091 [CDS_mKalama1_PartType9]
Ampicillin
Plate 1 / H / 9 pXTK092 [CDS_mTurquoise_PartType9]
Ampicillin
Plate 1 / H / 10 pXTK093 [CDS_YFP_PartType9]
Ampicillin
Plate 1 / H / 11 pXTK094 [Term_rrnB-T1_PartType7]
Ampicillin
Plate 1 / H / 12 pXTK095 [Term_bla-v1_PartType7]
Ampicillin
Plate 2 / A / 1 pXTK096 [Term_bla-v2_PartType7]
Ampicillin
Plate 2 / A / 2 pXTK097 [Term_cat_PartType7]
Ampicillin
Plate 2 / A / 3 pXTK098 [Term_nptII_PartType7]
Ampicillin
Plate 2 / A / 4 pXTK099 [Term_aacC1_PartType7]
Ampicillin
Plate 2 / A / 5 pXTK100 [Term_neo_PartType7]
Ampicillin
Plate 2 / A / 6 pXTK101 [Term_T0_PartType7]
Ampicillin
Plate 2 / A / 7 pXTK102 [Term_T1_PartType7]
Ampicillin
Plate 2 / A / 8 pXTK103 [Term_T7Te_PartType7]
Ampicillin
Plate 2 / A / 9 pXTK104 [Term_tetA_PartType7]
Ampicillin
Plate 2 / A / 10 pXTK105 [Term_tetR_PartType7]
Ampicillin
Plate 2 / A / 11 pXTK106 [Term_SynTer-v1-P2_PartType7]
Ampicillin
Plate 2 / A / 12 pXTK107 [Term_rrnB-T1_PartType10]
Ampicillin
Plate 2 / B / 1 pXTK108 [Term_bla-v1_PartType10]
Ampicillin
Plate 2 / B / 2 pXTK109 [Term_bla-v2_PartType10]
Ampicillin
Plate 2 / B / 3 pXTK110 [Term_cat_PartType10]
Ampicillin
Plate 2 / B / 4 pXTK111 [Term_nptII_PartType10]
Ampicillin
Plate 2 / B / 5 pXTK112 [Term_aacC1_PartType10]
Ampicillin
Plate 2 / B / 6 pXTK113 [Term_neo_PartType10]
Ampicillin
Plate 2 / B / 7 pXTK114 [Term_T0_PartType10]
Ampicillin
Plate 2 / B / 8 pXTK115 [Term_T1_PartType10]
Ampicillin
Plate 2 / B / 9 pXTK116 [Term_T7Te_PartType10]
Ampicillin
Plate 2 / B / 10 pXTK117 [Term_tetA_PartType10]
Ampicillin
Plate 2 / B / 11 pXTK118 [Term_tetR_PartType10]
Ampicillin
Plate 2 / B / 12 pXTK119 [Term_SynTer-v1-P2_PartType10]
Ampicillin
Plate 2 / C / 1 pXTK120 [Misc_oriT-IncP_PartType0]
Ampicillin
Plate 2 / C / 2 pXTK121 [Misc_CEN6-ARS4-HIS3_PartType11]
Ampicillin
Plate 2 / C / 3 pXTK122 [Misc_Expanding-Modulator-A_PartType8-11]
Ampicillin
Plate 2 / C / 4 pXTK123 [Misc_Expanding-Modulator-B_PartType8-11]
Ampicillin
Plate 2 / C / 5 pXTK124 [Misc_Terminating-Modulator-A_PartType8-11]
Ampicillin
Plate 2 / C / 6 pXTK125 [Misc_Terminating-Modulator-B_PartType8-11]
Ampicillin
Plate 2 / C / 7 pXTK126 [Linker_SynTer-L1_PartType5-11]
Ampicillin
Plate 2 / C / 8 pXTK127 [Linker_SynTer-L2_PartType8-11]
Ampicillin
Plate 2 / C / 9 pXTK128 [Linker_SynTer-L3_PartType11]
Ampicillin
Plate 2 / C / 10 pXTK129 [Linker_SynTer-L4_PartType8-10]
Ampicillin
Plate 2 / C / 11 pXTK130 [Linker_SynTer-L5_PartType5-10]
Ampicillin
Plate 2 / C / 12 pXTK131 [Linker_SynTer-L6_PartType3-4]
Ampicillin
Plate 2 / D / 1 pXTK132 [Linker_SynTer-L7_PartType3]
Ampicillin
Plate 2 / D / 2 pXTK133 [Linker_SynTer-L8_PartType4]
Ampicillin
Plate 2 / D / 3 pXTK134 [Linker_SynTer-L9_PartType0]
Ampicillin
Plate 2 / D / 4 pXTK135 [Linker_SynTer-L10_PartType8-0]
Ampicillin
Plate 2 / D / 5 pXTK136 [Linker_MCS-L1_PartType5-11]
Ampicillin
Plate 2 / D / 6 pXTK137 [Linker_MCS-L2_PartType8-11]
Ampicillin
Plate 2 / D / 7 pXTK138 [Linker_MCS-L3_PartType11]
Ampicillin
Plate 2 / D / 8 pXTK139 [Linker_MCS-L4_PartType8-10]
Ampicillin
Plate 2 / D / 9 pXTK140 [Linker_MCS-L5_PartType5-10]
Ampicillin
Plate 2 / D / 10 pXTK141 [Linker_MCS-L6_PartType3-4]
Ampicillin
Plate 2 / D / 11 pXTK142 [Linker_MCS-L7_PartType3]
Ampicillin
Plate 2 / D / 12 pXTK143 [Linker_MCS-L8_PartType4]
Ampicillin
Plate 2 / E / 1 pXTK144 [Linker_MCS-L9_PartType0]
Ampicillin
Plate 2 / E / 2 pXTK145 [Linker_MCS-L10_PartType8-0]
Ampicillin
Plate 2 / E / 3 pXTK146 [Origin_RK2-PaqCI-Compatible_PartType1]
Ampicillin
Plate 2 / E / 4 pXTK147 [Origin_RK2-PaqCI-Compatible_PartType3]
Ampicillin
Plate 2 / E / 5 pXTK148 [Marker_TetR-tetAR-PaqCI-Compatible_PartType2]
Ampicillin
Plate 2 / E / 6 pXTK149 [Marker_TetR-tetAR-PaqCI-Compatible_PartType4]
Ampicillin
Plate 2 / E / 7 pXTK-BB1 [Backbone_RK2-KanR_PartTypeBB]
Kanamycin
Plate 2 / E / 8 pXTK-BB2 [Backbone_RK2-KanR-TetR_PartTypeBB]
Kanamycin
Plate 2 / E / 9 pXTK-BB3 [Backbone_repABC-pMlb-KanR_PartTypeBB]
Kanamycin
Plate 2 / E / 10 pXTK-BB4 [Backbone_repABC-pMlb-KanR-TetR_PartTypeBB]
Kanamycin
Plate 2 / E / 11 pXTK-BB5 [Backbone_RK2-KanR-PaqCI-Compatible_PartTypeBB]
Kanamycin
Plate 2 / E / 12 pXTK-BB6 [Backbone_RK2-KanR-TetR-PaqCI-Compatible_PartTypeBB]
Kanamycin
Plate 2 / F / 1 pXTK-BB7 [Backbone_repABC-pMlb-KanR-TetR-PaqCI-Compatible_PartTypeBB]
Kanamycin
Plate 2 / F / 2 pXTK-Empty [Part Cloning Vector]
Ampicillin
Plate 2 / F / 3 pXTK151 [Term_BBa-B0015_PartType10]
Ampicillin
Plate 2 / F / 4 pXTK150 [Term_BBa-B0015_PartType7]
Ampicillin
Plate 2 / F / 5 Escherichia coli DH10B-MOB
60 ug/mL of DAP
Data cached @ 2025-10-31

Kit Plate Map - #1000000259

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