Skip to main content
Addgene

TCR Rapid Assembly for Functional Testing (TCRAFT) Libraries
(Pooled Library #1000000264)

Print
  • Purpose

    TCR Rapid Assembly for Functional Testing (TCRAFT) enables efficient assembly of paired αβ T-cell receptor (TCR) libraries from sequences by accurately linking TCR variable region genes to CDR3α/β-containing oligos through three Golden Gate reactions. The TCRAFT kit consists of four vector pools (1A, 1B, 2A, and 2B) encoding TCR components and a destination expression vector, pHIV-LacZ-SapI (Plasmid #237610).

    To build a TCR library, users generate two oligo pools with the TCRAFT script linked in the protocol and perform three simple Golden Gate assembly reactions with the four vector pools in this kit. The resulting TCR library can be expressed in cell lines or primary cells for functional screening.

  • Vector Backbone
    TRBV-TRAC Subpool 1A (#237606) and 1B (#237607): TRBC-TRAV Subpool 2A (#237608) and 2B (#237609):

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 1000000264 TCRAFT Libraries (4 tubes) + pHIV-LacZ-SapI (Plasmid #237610)
  • #237606 (TRBV-TRAC Subpool 1A)
  • #237607 (TRBV-TRAC Subpool 1B)
  • #237608 (TRBC-TRAV Subpool 2A)
  • #237609 (TRBC-TRAV Subpool 2B)
 1 $825 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Species
    Human
  • Number of plasmids
    • TRBV-TRAC Subpool 1A (#237606): 22
    • TRBV-TRAC Subpool 1B (#237607): 23
    • TRBC-TRAV Subpool 2A (#237607): 24
    • TRBC-TRAV Subpool 2B (#237609): 24
  • Size of insert
    ~900 bp

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼10 µL
  • Concentration
    20 ng/µL

Resource Information

Depositor Comments

Schematic of three Golden Gate reactions. The first reaction links pooled CDR3α/β-containing oligos to the TRBV-TRAC-containing αβ TCR libraries resulting in 48 vectors. The second reaction uses this product and links to 45 TRBC-TRAV vectors. This yields a complete TCR library that can be transferred to an expression vector of choice in a third reaction.

Figure 1: Human TCR library assembly.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human TCRAFT libraries were a gift from Michael Birnbaum (Addgene #1000000264)

  • For your References section:

    Scalable TCR synthesis and screening enables antigen reactivity mapping in vitiligo. Gaglione SA, Mukkamala RS, Krishna C, Smith BE, Wadsworth II MH, Jelinsky SA, Perez CR, Schmidt-Hong L, Katz EL, Gellatly KJ, Ali LR, Shen J, Holec PV, Zhao QH, Chan AO , Xu EJK, Kravarik KM, Guzova JA, Dobson CS, Singh H, Garber M, Dougan M, Dougan SK, Harris JE, Winkler A, Birnbaum ME. bioRxiv 2025.04.24.649726. doi: 10.1101/2025.04.24.649726. bioRxiv 2025.04.24.649726
Related items
Additional resources