pEIGW-human KLF4
(Plasmid #100131)

• Purpose: Human KLF4 lentiviral expression vector
• Depositing Lab: Eva Hernando
• Publication: Segura et al Oncotarget. 2017 Apr 18;8(16):26298-26311. doi: 10.18632/oncotarget.15459.

Backbone

• Vector backbone: pEIGW
• Backbone size w/o insert (bp): 10102
• Total vector size (bp): 11953
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Kruppel like factor 4
• Alt name: KLF4
• Species: H. sapiens (human)
• Insert Size (bp): 1851
• Entrez Gene: KLF4 (a.k.a. EZF, GKLF)
• Promoter: E1F

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (destroyed during cloning)
• 3′ cloning site: EcoRV (destroyed during cloning)
• 5′ sequencing primer: TGCCGCCAGA ACACAGGTG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry