sh-SRF
(Plasmid
#100797)
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Purposeexpression of shRNA targeting SRF
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 100797 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSilencer 3.0 H1
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Backbone manufacturerAmbion
- Backbone size w/o insert (bp) 2795
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Vector typeRNAi
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namerat SRF
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gRNA/shRNA sequenceCTGCAGCCCATGATCACCA
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SpeciesH. sapiens (human), R. norvegicus (rat)
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Insert Size (bp)65
- Promoter H1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer M13 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Conserved target in human and rat
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
sh-SRF was a gift from Joseph Gordon (Addgene plasmid # 100797 ; http://n2t.net/addgene:100797 ; RRID:Addgene_100797) -
For your References section:
A conserved MADS-box phosphorylation motif regulates differentiation and mitochondrial function in skeletal, cardiac, and smooth muscle cells. Mughal W, Nguyen L, Pustylnik S, da Silva Rosa SC, Piotrowski S, Chapman D, Du M, Alli NS, Grigull J, Halayko AJ, Aliani M, Topham MK, Epand RM, Hatch GM, Pereira TJ, Kereliuk S, McDermott JC, Rampitsch C, Dolinsky VW, Gordon JW. Cell Death Dis. 2015 Oct 29;6:e1944. doi: 10.1038/cddis.2015.306. 10.1038/cddis.2015.306 PubMed 26512955
