Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
We have implemented updates to our Transparency and Privacy Policy.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #100799)


Item Catalog # Description Quantity Price (USD)
Plasmid 100799 Standard format: Plasmid sent in bacteria as agar stab 1 $75
AAV1 100799-AAV1 Virus (100 µL at titer ≥ 1×10¹³ vg/mL) and Plasmid.

Currently unavailable outside the U.S.

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    mTagBFP2 and rabies virus SAD B19 strain glycoprotein genes (B19G)
  • Species
  • Insert Size (bp)
  • Promoter TREtight

Cloning Information

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Information for AAV1 (Catalog # 100799-AAV1) ( Back to top )


Ready-to-use AAV1 particles produced from pAAV-TREtight-mTagBFP2-B19G (#100799). In addition to the viral particles, you will also receive purified pAAV-TREtight-mTagBFP2-B19G plasmid DNA.

Helper virus for monosynaptic tracing with rabies virus; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA (Addgene# 100798). These AAV preparations are suitable purity for injection into animals.


  • Volume 100 µL
  • Titer ≥ 1×10¹³ vg/mL
  • Pricing $350 USD for preparation of 100 µL virus + $30 USD for plasmid.
  • Storage Store at -80℃. Thaw just before use and keep on ice.
  • Shipment Viral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

  • Packaging Plasmids encode adenoviral helper sequences and AAV rep gene, AAV1 cap gene
  • Buffer PBS + 0.001% Pluronic F-68
  • Serotype AAV1
  • Purification Iodixanol gradient ultracentrifugation
  • Reporter Gene BFP (in presence of Cre and 100798)


Requestor is responsible for compliance with their institution's biosafety regulations. Lentivirus is generally considered BSL-2. AAV is generally considered BSL-1, but may require BSL-2 handling depending on the insert. Biosafety Guide

Terms and Licenses

Viral Quality Control

Quality Control:
  • Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). The specific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
  • Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for more information.

Addgene Comments

Dr. Wickersham has tested these preps and he recommends the following dilution and mix:

  • pAAV-syn-FLEX-splitTVA-EGFP-tTA (Addgene #100798-AAV1, lot#v15287 (1.7 × 10^13 GC∕mL): 1:200 dilution, to be mixed 50/50 by volume with diluted 100799 below:
  • pAAV-TREtight-mTagBFP2-B19G (Addgene #100799-AAV1, lot#v14716 (3.2 × 10^13 GC∕mL): 1:20 dilution, to be mixed 50/50 by volume with diluted 100798 above;

When selecting what system to use, keep in mind that the two vector Addgene#100798 + Addgene# 100799 combination allows reduction of background infection by rabies virus in wild-type mice to acceptably few cells while still allowing nice amounts of transsynaptic spread in Cre+ mice (as compared to using Addgene#52473 alone). Note that EGFP can be seen only with immunostaining at these dilutions.

Dr. Wickersham recommends injecting the rabies virus 7 days after the AAVs and perfusing 7 days after that.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-TREtight-mTagBFP2-B19G was a gift from Ian Wickersham (Addgene plasmid # 100799 ; ; RRID:Addgene_100799)

    For viral preps, please replace (Addgene plasmid # 100799) in the above sentence with: (Addgene viral prep # 100799-AAV1)

  • For your References section:

    Lhx6-positive GABA-releasing neurons of the zona incerta promote sleep. Liu K, Kim J, Kim DW, Zhang YS, Bao H, Denaxa M, Lim SA, Kim E, Liu C, Wickersham IR, Pachinis V, Hattar S, Song J, Brown SP, Blackshaw S. Nature. 2017 Aug 31;548(7669):582-587. doi: 10.1038/nature23663. Epub 2017 Aug 23. 10.1038/nature23663 PubMed 28847002