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SGL40C.EFS.E2Crimson
(Plasmid #100894)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100894 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    SIN40C

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Screen multiple colonies to ensure that you have isolated the full, intact plasmid.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E2Crimson
  • gRNA/shRNA sequence
    gRNA (empty)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this plasmid is prone to recombination at the LTR sites. We recommend screening multiple colonies to ensure that you have isolated the full, intact plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SGL40C.EFS.E2Crimson was a gift from Dirk Heckl (Addgene plasmid # 100894 ; http://n2t.net/addgene:100894 ; RRID:Addgene_100894)

  • For your References section:

    Refined sgRNA efficacy prediction improves large- and small-scale CRISPR-Cas9 applications. Labuhn M, Adams FF, Ng M, Knoess S, Schambach A, Charpentier EM, Schwarzer A, Mateo JL, Klusmann JH, Heckl D. Nucleic Acids Res. 2018 Feb 16;46(3):1375-1385. doi: 10.1093/nar/gkx1268. 10.1093/nar/gkx1268 PubMed 29267886
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