FL tension sensor module
(Plasmid #101170)

• Purpose: Expresses the FL-based tension sensor module. The biosensor allows force measurements at 3-5 pN.
• Depositing Lab: Carsten Grashoff
• Publication: Ringer et al Nat Methods. 2017 Nov;14(11):1090-1096. doi: 10.1038/nmeth.4431. Epub 2017 Sep 18.

Backbone

• Vector backbone: pLPCX
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 6238
• Total vector size (bp): 7936
• Modifications to backbone: modified multi cloning site
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: YPet(short)-FL-mCherry
• Alt name: FL tension sensor module
• Insert Size (bp): 1650
• Promoter: CMV
• Tags / Fusion Proteins:
  • cysteine (N terminal on insert)
  • cysteine (C terminal on insert)
  • His-tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: AGCTCGTTTAGTGAACCGTCAGATC
• 3′ sequencing primer: ACCTACAGGTGGGGTCTTTCATTCCC

Resource Information

• Supplemental Documents:
  • pLPCXmod + Cys-[YPet(short)-FL-mCherry]-Cys-His(tag).gb
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry