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FL tension sensor module
(Plasmid #101170)


Item Catalog # Description Quantity Price (USD)
Plasmid 101170 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 6238
  • Total vector size (bp) 7936
  • Modifications to backbone
    modified multi cloning site
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy


  • Gene/Insert name
  • Alt name
    FL tension sensor module
  • Insert Size (bp)
  • Promoter CMV
  • Tags / Fusion Proteins
    • cysteine (N terminal on insert)
    • cysteine (C terminal on insert)
    • His-tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer AGCTCGTTTAGTGAACCGTCAGATC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FL tension sensor module was a gift from Carsten Grashoff (Addgene plasmid # 101170 ; ; RRID:Addgene_101170)
  • For your References section:

    Multiplexing molecular tension sensors reveals piconewton force gradient across talin-1. Ringer P, Weissl A, Cost AL, Freikamp A, Sabass B, Mehlich A, Tramier M, Rief M, Grashoff C. Nat Methods. 2017 Nov;14(11):1090-1096. doi: 10.1038/nmeth.4431. Epub 2017 Sep 18. 10.1038/nmeth.4431 PubMed 28945706