|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||102244||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9651
Vector typeMammalian Expression, Lentiviral, CRISPR
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberLow Copy
SpeciesH. sapiens (human)
Insert Size (bp)5169
- Promoter EF1a
/ Fusion Protein
- HA-TagBFP-KRAB-NLS (C terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Contains HA tag and TagBFP fusion. The plasmid is derived from Addgene Plasmid #46911. The promoter was changed to human EF1a and the NLS was moved to the C-terminus of the fusion protein.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHR-EF1a-dCas9-HA-BFP-KRAB-NLS was a gift from Jacob Corn (Addgene plasmid # 102244 ; http://n2t.net/addgene:102244 ; RRID:Addgene_102244)