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CRY2low-mCherry-Raf1
(Plasmid #104066)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 104066 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5300
  • Total vector size (bp) 9500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CRY2low-mCherry-Raf1
  • Alt name
    CRY2PHR
  • Alt name
    mCherry
  • Alt name
    Raf1
  • Species
    H. sapiens (human), A. thaliana (mustard weed)
  • Mutation
    CRY2 aa 1-491 truncation, R489E A491D
  • Entrez Gene
    CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
  • Entrez Gene
    RAF1 (a.k.a. CMD1NN, CRAF, NS5, Raf-1, c-Raf)
  • Promoter CMV

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CRY2low-mCherry-Raf1 was a gift from Bianxiao Cui (Addgene plasmid # 104066 ; http://n2t.net/addgene:104066 ; RRID:Addgene_104066)
  • For your References section:

    Understanding CRY2 interactions for optical control of intracellular signaling. Duan L, Hope J, Ong Q, Lou HY, Kim N, McCarthy C, Acero V, Lin MZ, Cui B. Nat Commun. 2017 Sep 15;8(1):547. doi: 10.1038/s41467-017-00648-8. 10.1038/s41467-017-00648-8 [pii] PubMed 28916751