UNC50-myc
(Plasmid #104382)

• Purpose: Expresses UNC50-myc wild-type in mammalian cells
• Depositing Lab: Somshuvra Mukhopadhyay
• Publication: Selyunin et al J Cell Biol. 2017 Oct 2;216(10):3249-3262. doi: 10.1083/jcb.201704015. Epub 2017 Sep 7.

Backbone

• Vector backbone: pEGFP-N3
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5500
• Modifications to backbone: Stop codon introduced after UNC50 insert and before start of GFP sequence. This plasmid does not express GFP. There is a myc tag at the N-terminus of the UNC50 insert.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: UNC50
• Species: H. sapiens (human)
• Insert Size (bp): 777
• Entrez Gene: UNC50 (a.k.a. GMH1, HSD23, PDLs22, UNCL, URP)
• Promoter: CMV
• Tag / Fusion Protein:
  • myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: AAC TCG AGA TGG AAC AAA AAC TCA TCT CAG AAG AGG ATC TGA TGT TAC CGA GTA CTT CAG
• 3′ sequencing primer: AAG GAT CCT TAT TTC ACT CTG TAC TTA TAG AAA GAAC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry