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UNC50-myc
(Plasmid #104382)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104382 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 104382-D50 50 μg of DNA in Tris buffer $495
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DNA 104382-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEGFP-N3
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5500
  • Modifications to backbone
    Stop codon introduced after UNC50 insert and before start of GFP sequence. This plasmid does not express GFP. There is a myc tag at the N-terminus of the UNC50 insert.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UNC50
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    777
  • Entrez Gene
    UNC50 (a.k.a. GMH1, HSD23, PDLs22, UNCL, URP)
  • Promoter CMV
  • Tag / Fusion Protein
    • myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer AAC TCG AGA TGG AAC AAA AAC TCA TCT CAG AAG AGG ATC TGA TGT TAC CGA GTA CTT CAG
  • 3′ sequencing primer AAG GAT CCT TAT TTC ACT CTG TAC TTA TAG AAA GAAC
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UNC50-myc was a gift from Somshuvra Mukhopadhyay (Addgene plasmid # 104382 ; http://n2t.net/addgene:104382 ; RRID:Addgene_104382)

  • For your References section:

    Genome-wide siRNA screen identifies UNC50 as a regulator of Shiga toxin 2 trafficking. Selyunin AS, Iles LR, Bartholomeusz G, Mukhopadhyay S. J Cell Biol. 2017 Oct 2;216(10):3249-3262. doi: 10.1083/jcb.201704015. Epub 2017 Sep 7. 10.1083/jcb.201704015 PubMed 28883040
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