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Addgene

p-dCas9-TET1-Hygro
(Plasmid #104404)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104404 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMLM3705
  • Backbone manufacturer
    Keith Joung (Addgene plasmid # 47754)
  • Backbone size w/o insert (bp) 9075
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TET1
  • Species
    H. sapiens (human)
  • Entrez Gene
    TET1 (a.k.a. CXXC6, LCX, bA119F7.1)
  • Promoter CMV promoter

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Backbone modification: added Hygromycin resistance cassette (PGK promoter and Hygromycin resistance gene from Addgene Plasmid 41721 (MSCVhygro- F-G9a), SV40 early polyadenylation signal from the pCDNA3.1 backbone of Addgene Plasmid 13820 (HDAC1 Flag). TET1 catalytic domain (amino acids 1418- 2136) was from Addgene plasmid 39454 (pAAV-EF1a-HA-hTet1CD- WPRE-PolyA).

Use of this plasmid is described in:
Baumann, V., Wiesbeck, M., Breunig, C. T., Braun, J. M., Köferle, A., Ninkovic, J., et al. (2019). Targeted removal of epigenetic barriers during transcriptional reprogramming. Nature Communications, 1–12. http://doi.org/10.1038/s41467-019-10146-8

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p-dCas9-TET1-Hygro was a gift from Stephan Beck (Addgene plasmid # 104404 ; http://n2t.net/addgene:104404 ; RRID:Addgene_104404)