pPBbsr2-4031NES
(Plasmid #105241)

• Purpose: A Förster resonance energy transfer (FRET) biosensor for AMPK activity with cyan and yellow fluorescent protein.
• Depositing Lab: Michiyuki Matsuda
• Publication: Konagaya et al Cell Rep. 2017 Nov 28;21(9):2628-2638. doi: 10.1016/j.celrep.2017.10.113.

Backbone

• Vector backbone: pPB
• Backbone size w/o insert (bp): 6800
• Total vector size (bp): 9116
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: AMPK-EV
• Species: S. cerevisiae (budding yeast), Synthetic; aequorea coerulescens
• Insert Size (bp): 2300
• GenBank ID:
• Promoter: CAGGS

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: pCAGGS-5'
• 3′ sequencing primer: IRES-reverse

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry