pDest-Tol2 (Chien)-Tol2-Isl2b:GFP:polyA
(Plasmid #105648)

• Purpose: Expression of GFP in sensory neurons (retinal ganglion cells, trigeminal, Rohon Beard neurons and dorsal root ganglia) for up to 2 weeks
• Depositing Lab: Sandra Rieger
• Depositing Organization: Mount Desert Island Biological Laboratory
  Located in United States of America
• Publication: Rieger lab plasmids (unpublished)
• Citations: 1 Article

Backbone

• Vector backbone: pDESTTol2pA2
• Backbone manufacturer: Chi-Bin Chien lab
• Backbone size w/o insert (bp): 5800
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Tol2-Isl2b:GFP:polyA
• Alt name: isl3
• Species: D. rerio (zebrafish)
• Insert Size (bp): 2000
• Entrez Gene: isl2b (a.k.a. ISL-3, isl3, islet 3, zfIsl-3, zgc:109840)

Cloning Information

• Cloning method: Gateway Cloning

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Derived from two plasmids from Chi-Bin Chien’s lab at the University of Utah.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene NGS results found additional amino acids GGGRSGNSRPLEPLDSAAL at the C-terminus of the EGFP translation. Plasmid was validated and confirmed to function as intended by the depositing laboratory

DNA (Catalog # 105648-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 105648-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.