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pDest-Tol2 (Chien)-Tol2-Isl2b:GFP:polyA
(Plasmid #105648)


Item Catalog # Description Quantity Price (USD)
Plasmid 105648 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Chi-Bin Chien lab
  • Backbone size w/o insert (bp) 5800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
  • Entrez Gene
    isl2b (a.k.a. ISL-3, isl3, islet 3, zfIsl-3, zgc:109840)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Derived from two plasmids from Chi-Bin Chien’s lab at the University of Utah.

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS results found additional amino acids GGGRSGNSRPLEPLDSAAL at the C-terminus of the EGFP translation. Plasmid was validated and confirmed to function as intended by the depositing laboratory

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDest-Tol2 (Chien)-Tol2-Isl2b:GFP:polyA was a gift from Sandra Rieger (Addgene plasmid # 105648 ; ; RRID:Addgene_105648)