Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pAID 1.1 N-T2A-Bsr
(Plasmid #105985)


Item Catalog # Description Quantity Price (USD)
Plasmid 105985 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 7438
  • Total vector size (bp) 7577
  • Modifications to backbone
    insertion of T2A-Bsr selection marker instead of 9myc tag
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418), Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    E.coli growth is slow
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
  • Insert Size (bp)
  • GenBank ID
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAID 1.1 N-T2A-Bsr was a gift from Tatsuo Fukagawa (Addgene plasmid # 105985 ; ; RRID:Addgene_105985)
  • For your References section:

    An efficient method to generate conditional knockout cell lines for essential genes by combination of auxin-inducible degron tag and CRISPR/Cas9. Nishimura K, Fukagawa T. Chromosome Res. 2017 Oct;25(3-4):253-260. doi: 10.1007/s10577-017-9559-7. Epub 2017 Jun 6. 10.1007/s10577-017-9559-7 [pii] PubMed 28589221