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pAID 1.1 N-T2A-Bsr
(Plasmid #105985)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105985 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 105985-D50 50 μg of DNA in Tris buffer $495
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DNA 105985-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pAID1.1-N
  • Backbone size w/o insert (bp) 7438
  • Total vector size (bp) 7577
  • Modifications to backbone
    insertion of T2A-Bsr selection marker instead of 9myc tag
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418), Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    E.coli growth is slow
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    T2A-Bsr
  • Species
    Synthetic
  • Insert Size (bp)
    477
  • GenBank ID
  • Promoter CMV

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAID 1.1 N-T2A-Bsr was a gift from Tatsuo Fukagawa (Addgene plasmid # 105985 ; http://n2t.net/addgene:105985 ; RRID:Addgene_105985)

  • For your References section:

    An efficient method to generate conditional knockout cell lines for essential genes by combination of auxin-inducible degron tag and CRISPR/Cas9. Nishimura K, Fukagawa T. Chromosome Res. 2017 Oct;25(3-4):253-260. doi: 10.1007/s10577-017-9559-7. Epub 2017 Jun 6. 10.1007/s10577-017-9559-7 [pii] PubMed 28589221
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