1553 pcDNA3 HA gamma adaptin 2
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10713||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5446
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameGamma adaptin 2
SpeciesH. sapiens (human)
Insert Size (bp)2300
Entrez GeneAP1G2 (a.k.a. G2AD)
/ Fusion Protein
- HA (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SalI/XhoI (destroyed during cloning)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
diagnostic digest with BamHI should release 800bp fragment.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:1553 pcDNA3 HA gamma adaptin 2 was a gift from William Sellers (Addgene plasmid # 10713 ; http://n2t.net/addgene:10713 ; RRID:Addgene_10713)
Map uploaded by the depositor.