pSP64T-hLbCpf1
(Plasmid
#107187)
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Purposeplasmid to carry out IVT of human codon optimized LbCpf1 (based on Addgene 69988)
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Depositing Lab
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Depositing OrganizationYale University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 107187 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 107187-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 107187-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepSP64T
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehLbCpf1
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Alt nameLbCas12a, LbCpf1
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SpeciesH. sapiens (human); Lachnospiraceae_bacterium ND2006
- Promoter sp6
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Tag
/ Fusion Protein
- NLS-3xHA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site EcorRI (not destroyed)
- 5′ sequencing primer sp6
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/156125 for bioRxiv preprint.
DNA (Catalog # 107187-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 107187-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSP64T-hLbCpf1 was a gift from Antonio Giraldez (Addgene plasmid # 107187 ; http://n2t.net/addgene:107187 ; RRID:Addgene_107187) -
For your References section:
CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. Moreno-Mateos MA, Fernandez JP, Rouet R, Vejnar CE, Lane MA, Mis E, Khokha MK, Doudna JA, Giraldez AJ. Nat Commun. 2017 Dec 8;8(1):2024. doi: 10.1038/s41467-017-01836-2. 10.1038/s41467-017-01836-2 [pii] PubMed 29222508
