VGluT1-SNAPtag
(Plasmid #107371)

• Purpose: Synaptic vesicle protein VGluT1 fused to the SNAPtag domain
• Depositing Lab: David Perrais
• Depositing Organization: CNRS UMR 5297, Institut Interdisciplinaire de Neurosciences (IINS)
  Located in France
• Publication: Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi: 10.1038/s41467-017-01752-5.

Backbone

• Vector backbone: pCaggs
• Backbone size w/o insert (bp): 2361
• Total vector size (bp): 7347
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Vesicular Glutamate transporter 1
• Alt name: BNPI
• Alt name: Slc17a7
• Species: R. norvegicus (rat)
• Entrez Gene: Slc17a7 (a.k.a. BNPI, Vglut1)
• Tag / Fusion Protein:
  • SNAPtag, 104 aa before C term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

DNA (Catalog # 107371-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 107371-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.