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ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP)
(Plasmid #109428)


Item Catalog # Description Quantity Price (USD)
Plasmid 109428 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    HIV-1 NL4-3
  • Backbone size w/o insert (bp) 9269
  • Total vector size (bp) 10814
  • Modifications to backbone
    Removed HIV-1 nef, vif, vpr, and part of env. Inserted CMV mCherry T2A GFP with a 43 base pair insertion in mCherry.
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number


  • Gene/Insert name
    mCherry T2A GFP
  • Species
  • Insert Size (bp)
  • Mutation
    Inserted 43 base pairs into mCherry to create reporter
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer ctggctaccggtatggtgagcaagggcgagg
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP) was a gift from Reuben Harris (Addgene plasmid # 109428 ; ; RRID:Addgene_109428)
  • For your References section:

    A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC-Cas9 or cleavage by Cas9 in living cells. St Martin A, Salamango D, Serebrenik A, Shaban N, Brown WL, Donati F, Munagala U, Conticello SG, Harris RS. Nucleic Acids Res. 2018 May 9. pii: 4994269. doi: 10.1093/nar/gky332. 10.1093/nar/gky332 PubMed 29746667