|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11010||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepYC6-CT/LYS2 hybrid
- Backbone size w/o insert (bp) 7374
Vector typeBacterial Expression, Yeast Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesS. cerevisiae (budding yeast)
Insert Size (bp)2240
MutationORF-TRP1 fused to ORF-URA without stop codon
/ Fusion Proteins
- his6 (C terminal on backbone)
- V5 (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NaeI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Please note that Addgene's sequencing results identified mutations A160S and K173R in the URA3 sequence. These mutations were not intentional and the depositing scientist states that the plasmid was tested for complementation of URA and TRP phenotypes and for production of a single transcript.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pYC6Lys-TRP1URA3 was a gift from Francisco Malagon (Addgene plasmid # 11010 ; http://n2t.net/addgene:11010 ; RRID:Addgene_11010)