pQC MCS IRES G418
(Plasmid #110344)

• Purpose: (Empty Backbone) γ-Retroviral transfer vector for cloning and expressing your gene of interest. IRES-driven Geneticin selection.
• Depositing Lab: Sandra Martha Gomes Dias
• Publication: Mammalian retroviral expression plasmids based on pQC family (unpublished)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	110344	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pQC MCS IRES Puro
  (Search Vector Database)
• Backbone manufacturer: #110343
• Backbone size (bp): 7067
• Modifications to backbone: Region between BglII and EcoRI from original backbone replaced by same region from pQCXI Neo DsRed-LC3-GFP (Addgene #31183).
• Vector type: Mammalian Expression, Retroviral
• Promoter: CMV
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pQC Seq Fow 5'-ACGCCATCCACGCTGTTTTGACCT-3'
• 3′ sequencing primer: pQC Seq Rev 5'-AAGCGGCTTCGGCCAGTAACGTTA-3'

Resource Information

• Supplemental Documents:
  • pD015-pQC MCS IRES G418.gb
• A portion of this plasmid was derived from a plasmid made by: Used sequences from pQC mKorange IX (Addgene #37344), Dr. Connie Cepko, pLKO.1 - TRC cloning vector (Addgene #10878), Dr. David Root and pQCXI Neo DsRed-LC3-GFP (Addgene #31183), Dr. David Sabatini
• Terms and Licenses:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pQC-based γ-Retroviral transfer vector for cloning and expressing your gene of interest. IRES-driven Geneticin selection.

Also available as:
V5-mKO2-MCS-IRES-Puro (Addgene #110341)
V5 MCS IRES Puro (Addgene #110342)
MCS IRES Puro (Addgene #110343)
MCS IRES G418 (Addgene #110344)
V5 MCS IRES G418 (Addgene #110345)

All vectors can be used for TA-cloning (After XcmI cleavage), or BamHI-AgeI-PmeI-EcoRI restriction cloning.

Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging.