pGEMT-Mef2c-tPT2A-GFP
(Plasmid #111771)

• Purpose: Bicistronic construct: Co-express two genes by 2A peptides
• Depositing Lab: Li Qian
• Publication: Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.1038/s41598-017-02460-2.

Backbone

• Vector backbone: pGEMT-easy
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 5100
• Modifications to backbone: Original MCS removed
• Vector type: Cloning intermediate

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mef2c-tPT2A-GFP
• Species: M. musculus (mouse)
• Insert Size (bp): 2200
• Entrez Gene: Mef2c (a.k.a. 5430401D19Rik, 9930028G15Rik, Mef2)
• Promoter: T7
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: M13 Forward/T7
• 3′ sequencing primer: M13 Rev/SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that several discrepancies were found between Addgene's quality control and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

If co-express two genes connected by solely P2A or T2A is desired rather than the tandom P2A-T2A here, just use available restriction enzymes on the vector. Please refer to the uploaded plasmid sequence and the published article for details.