PurposeBicistronic construct: Co-express two genes by 2A peptides
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||111771||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 5100
Modifications to backboneOriginal MCS removed
Vector typeCloning intermediate
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)2200
Entrez GeneMef2c (a.k.a. 5430401D19Rik, 9930028G15Rik, AV011172, Mef2)
- Promoter T7
/ Fusion Protein
- GFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer M13 Forward/T7
- 3′ sequencing primer M13 Rev/SP6 (Common Sequencing Primers)
Terms and Licenses
Please note that several discrepancies were found between Addgene's quality control and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
If co-express two genes connected by solely P2A or T2A is desired rather than the tandom P2A-T2A here, just use available restriction enzymes on the vector. Please refer to the uploaded plasmid sequence and the published article for details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGEMT-Mef2c-tPT2A-GFP was a gift from Li Qian (Addgene plasmid # 111771 ; http://n2t.net/addgene:111771 ; RRID:Addgene_111771)
For your References section:Systematic comparison of 2A peptides for cloning multi-genes in a polycistronic vector. Liu Z, Chen O, Wall JBJ, Zheng M, Zhou Y, Wang L, Ruth Vaseghi H, Qian L, Liu J. Sci Rep. 2017 May 19;7(1):2193. doi: 10.1038/s41598-017-02460-2. 10.1038/s41598-017-02460-2 PubMed 28526819