PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR1D2
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||112410||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerZhang lab (Addgene plasmid ID: 42230)
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
gRNA/shRNA sequenceGCCTTAAGGGTGAACTTTAA AGG
SpeciesH. sapiens (human)
Entrez GeneNR1D2 (a.k.a. BD73, EAR-1R, REVERBB, REVERBbeta, RVR)
- Cloning method Restriction Enzyme
- 5′ cloning site BbsI (destroyed during cloning)
- 3′ cloning site BbsI (destroyed during cloning)
- 5′ sequencing primer hU6-F (5'-GAGGGCCTATTTCCCATGATT-3') (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pNR1D2-donor.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pNR1D2.1.0-gDNA was a gift from Kevin White (Addgene plasmid # 112410 ; http://n2t.net/addgene:112410 ; RRID:Addgene_112410)